重组人血小板生成素可能通过调节免疫负调控因子水平治疗免疫性血小板减少性紫癜  

The Effects on Immune System in Patients with Immune Thrombocytopenic Treated by Recombinant Human Thrombopoietin

作　　者：陶媛 吴秉毅[2] 杜庆锋[3] 孙璨[2] 林遐[2] 黄宇贤[2] 涂三芳[2] 宋朝阳[2] 陈土珍[2] 

机构地区：[1]广州市番禺区中心医院血液科,广州511400 [2]南方医科大学珠江医院血液科 [3]南方医科大学管理中心

出　　处：《血栓与止血学》2014年第6期315-318,共4页Chinese Journal of Thrombosis and Hemostasis

摘　　要：目的观察使用重组人血小板生成素(rh TPO,特比澳)前后免疫性血小板减少性紫癜(ITP)患者血浆IL-10,CTLA-4,TGF-β1变化情况,并与肾上腺皮质激素治疗前后ITP患者以及正常人血浆IL-10,CTLA-4,TGF-β1含量的比较。方法采用酶联免疫吸附试验(ELISA)测定21例ITP患者(其中11例使用rh TPO治疗,10例使用肾上腺皮质激素治疗)治疗前、后及15名正常对照者血浆IL-10,CTLA-4,TGF-β1水平,并对检测结果进行分析。结果 ITP患者血浆中IL-10、CTLA-4、TGF-β1水平明显低于正常人;经rh TPO或肾上腺皮质激素治疗后IL-10,CTLA-4,TGF-β1水平均有明显升高,并恢复至正常人水平;rh TPO及肾上腺皮质激素升高ITP患者血浆中IL-10,CTLA-4,TGF-β1水平的程度无差别。结论 ITP患者IL-10,CTLA-4,TGF-β1水平低于正常人,经治疗后水平可恢复正常;单用rh TPO后三种细胞因子水平显著升高,提示rh TPO可能有免疫调节的作用。Objective To investigate the changes of the levels of plasma contents of 3 cytokines（IL-10,CTLA-4,TGF-β1）of immune thrombocytopenic purpura （ITP）patients treated by recombinant human thrombopoietin （rhTPO） or steroid.Methods We use Enzyme-linked immunosorbent assay （ELISA） to measured the levels of 3 cytokines of 21 ITP patients（11 patients was treated by rhTPO,10 patients was treated by steroid） before and after the treatment,and 15 healthy people were measured as control.Results The levels for ITP patient＇ s plasma contents of both 3 cytokines were significantly lower than normal ; after the treatment of rhTPO or steroid,levels were significantly higher,and recovery to normal levels.Conclusion The levels of plasma contents of 3 cytokines （IL-10,CTLA-4,TGF-β1） of ITP patients were lower than normal,and return to normal levels after treatment; the use of rhTPO can significantly increased the levels of 3 cytokines,suggesting that rhTPO may modulate immune system.

关 键 词：免疫性血小板减少性紫癜 重组人血小板生成素 白细胞介素10 细胞毒T淋巴细胞相关抗原4 转化生长因子Β1 

分 类 号：R558[医药卫生—血液循环系统疾病]