氟伐他汀对尼古丁诱导血管内皮功能障碍的保护作用  

Protective effect of fluvastatin on nicotine-induced endothelial dysfunction

作　　者：胡翠竹[1] 蔡冬梅[1] 胡健[1] 

机构地区：[1]中国医科大学附属第一医院,沈阳110001

出　　处：《实用药物与临床》2014年第11期1414-1416,共3页Practical Pharmacy and Clinical Remedies

基　　金：高等学校博士学科点专项科研基金(20112104120002)

摘　　要：目的观察尼古丁对人脐静脉内皮细胞白介素(IL)-8表达的影响,及氟伐他汀对尼古丁诱导人脐静脉内皮细胞表达的干预作用,以及这一过程中核转录因子(NF)-κB的调节机制。方法采用人脐静脉内皮细胞株传代培养,细胞随机分为4组,空白对照组:无血清DMEM培养基代替干预因素;尼古丁组:在无血清培养基中加入100 nmol/L尼古丁孵育内皮细胞24 h;氟伐他汀组:在培养基中10-5mol/L氟伐他汀孵育内皮细胞1 h后,100 nmol/L尼古丁孵育内皮细胞24 h;NF-κB抑制剂组:100μmol/L PDTC和100 nmol/L尼古丁孵育内皮细胞24 h,收集细胞标本及培养液。ELISA法检测内皮细胞培养液中IL-8的蛋白表达水平,Trans AM(tm)NF-κBp50检测试剂盒检测细胞NF-κB的活性。结果尼古丁组NF-κB活性较空白对照组明显升高(P<0.05);氟伐他汀组NF-κB活性较尼古丁组显著下降(P<0.05)。NF-κB抑制剂组较尼古丁组IL-8的蛋白表达明显下降(P<0.05),氟伐他汀组较尼古丁组IL-8的蛋白表达明显下降(P<0.05)。结论尼古丁可通过激活人脐静脉内皮细胞NF-κB的活性,从而诱导IL-8的表达;氟伐他汀可拮抗尼古丁诱发的NF-κB的活化,抑制尼古丁诱导的IL-8的表达,还可拮抗尼古丁引起的内皮功能紊乱,改善内皮细胞功能。Objective To investigate the effect of nicotine on human umbilical vein endothelial cell （ HU-VEC）,and to study the intervention of fluvastatin on nicotine induced interleukin-8（IL-8）expression and the possible mechanism associated with transduction factor NF-κB. Methods Human umbilical vein endothelial cell line was incu-bated with serum free DMEM medium and the cells were randomly separated to 4 groups：control group, nicotine group,fluvastatin group and NF-κB inhibitor group. The expression of IL-8 was determined by specific enzyme-linked immuno-sorbent assay（ ELISA） method. The NF-κB activity of HUVEC was detected by Trans AMTM NF-κB p50 Ac-tivity Assay. Results The activity of NF-κB：nicotine group was higher than control group （ P 〈0. 05 ）;fluvastatin group were lower than nicotine group（P〈0. 05）. Expression of IL-8：Both NF-κB inhibitor group and fluvastatin group were lower than nicotine group（P〈0. 05）. Conclusion Nicotine induces expression of IL-8 in HUVECs to provoke endothelium dysfunction. Fluvastatin can antagonize nicotine evoked NF-κB activity and inhibit the expression of IL-8 in-duced by nicotine. Fluvastatin plays an important role in improving endothelial function,and inhibiting nicotine-induced atherosclerosis.

关 键 词：氟伐他汀 尼古丁 血管内皮功能障碍 

分 类 号：R54[医药卫生—心血管疾病]