Influence of Allelochemicals on Microbial Community in Ginseng Cultivating Soil  

作　　者：Yong Li Yi-xin Ying Dong-yue Zhao Wan-long Ding 

机构地区：[1]Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College [2]National Resource Centre of Chinese Materia Medica,China Academy of Chinese Medical Sciences

出　　处：《Chinese Herbal Medicines》2014年第4期313-318,共6页中草药（英文版）

基　　金：National Nature Sciences Fund (81072992,81373911);Doctoral Fund of Ministry of Education of China (200800231060)

摘　　要：Objective To study the influence of allelochemicals such as benzoic acid, diisobutyl phthalate, diisobutyl succinate, palmitic acid, and 2,2-bis-（4-hydroxyphenyl） propane on the microbial community of ginseng cultivating soil. Methods Soil samples were sprayed with five allelochemicals（100 mg/L） and their mixture（20 mg/L）, respectively. Carbon metabolic ability variances were analyzed by Biolog method and genetic polymorphism variance was analyzed by RAPD method. The Nei＇s genetic diversity index and Shannon＇s information index were calculated. Results Significant differences in carbon metabolic activity were found between allelochemical-treated soils and control. Further analysis indicated that although the carbon-utilizing ability and genetic polymorphism of soils treated with di-isobutyl phthalate, di-isobutyl succinate, and allelochemical mixtures were lower than those of the other treatments, genetic similarities of soils treated with di-isobutyl phthalate, diisobutyl succinate, and allelochemical mixtures were much higher than those of the other treatments. Conclusion Allelochemicals significantly declined the genetic diversity and carbon metabolic activity of microorganisms in newly reclaimed forest soil for ginseng cultivation.Objective To study the influence of allelochemicals such as benzoic acid, diisobutyl phthalate, diisobutyl succinate, palmitic acid, and 2,2-bis-（4-hydroxyphenyl） propane on the microbial community of ginseng cultivating soil. Methods Soil samples were sprayed with five allelochemicals（100 mg/L） and their mixture（20 mg/L）, respectively. Carbon metabolic ability variances were analyzed by Biolog method and genetic polymorphism variance was analyzed by RAPD method. The Nei＇s genetic diversity index and Shannon＇s information index were calculated. Results Significant differences in carbon metabolic activity were found between allelochemical-treated soils and control. Further analysis indicated that although the carbon-utilizing ability and genetic polymorphism of soils treated with di-isobutyl phthalate, di-isobutyl succinate, and allelochemical mixtures were lower than those of the other treatments, genetic similarities of soils treated with di-isobutyl phthalate, diisobutyl succinate, and allelochemical mixtures were much higher than those of the other treatments. Conclusion Allelochemicals significantly declined the genetic diversity and carbon metabolic activity of microorganisms in newly reclaimed forest soil for ginseng cultivation.

关 键 词：allelochemicals Biolog Panax ginseng RAPD soil microorganisms 

分 类 号：S567.51[农业科学—中草药栽培] S154.3[农业科学—作物学]