搜风祛痰法对ApoE基因敲除小鼠过氧化物酶体增殖物激活受体γ表达的影响  

Effect of Searching Wind and Removing Phlegm Method on Peroxisome Proliferator-activated Receptor-γ Expression of ApoE-gene Knockout Mice

作　　者：李颖[1] 宫丽鸿[2] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中国中医急症》2014年第11期1968-1969,1983,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：中国博士后科学基金面上项目(20110491540);辽宁省"百千万人才工程"资助项目(2011921022)

摘　　要：目的通过观察过氧化物酶体增殖物激活受体γ(PPAR-γ)的表达水平,来阐明搜风祛痰法中药对ApoE基因敲除小鼠动脉粥样硬化不稳定斑块的干预作用机制。方法制备ApoE基因敲除小鼠动脉粥样硬化模型,随机分为5组:模型组、稳斑汤低剂量组、中剂量组、高剂量组及西药(他汀)组,另设空白对照组。采用HE染色、Masson染色法用于实验观察,应用RT-PCR法检测PPAR-γmRNA的表达。结果与空白组、模型组相比,稳斑汤各剂量组及西药组PPAR-γ mRNA表达水平明显升高(P<0.01)。结论稳斑汤可以提高PPAR-γmRNA表达水平,对小鼠动脉粥样硬化有一定稳定斑块的效应,因剂量不同而各异。Objective：To observe PPAR-γ expression level and to clarify the intervention mechanism of searching wind and removing phlegm method of traditional Chinese medicine on unstable atherosclerotic plaque of ApoE knockout mice.Methods：ApoE knockout mice with AS were randomly divided into 5 groups including model group,Wenban Decoction low dose group,Wenban Decoction middle dose group,Wenban Decoction high dose group,and the western medicine statin group.The pathological examinations such as HE staining and Masson staining method were used.Expressions of PPAR-γmRNA were detected by RT-PCR.Results：Compared with the blank group and model group,PPAR-γ mRNA expression level in traditional Chinese medicine dose groups and western medicine group increased significantly（P〈0.01）.Conclusion：Unstable plaque decoction can improve PPAR-γmRNA expression level,and has the effect of stable plaques with different dose.

关 键 词：搜风祛痰法 APOE基因敲除 小鼠 动脉粥样硬化 不稳定斑块 过氧化物酶体增殖物激活受体Γ 

分 类 号：R285.5[医药卫生—中药学]