高脂饲料对长爪沙鼠血脂和血清电解质的影响  

The Effect of High-fat Diet on Serum Lipid Parameters and Electrolyte Values in Mongolian Gerbils

作　　者：李雪枫[1] 王伊楠 金丹丹[1] 林东子 丁航[1] 张志珍[1] 马卫列[1] 

机构地区：[1]广东医学院基础医学院,广东东莞523808 [2]广东医学院第二临床医学院,广东东莞523808 [3]东莞市慢性病防治院检验科,广东东莞523008

出　　处：《广东微量元素科学》2014年第11期33-36,共4页Trace Elements Science

基　　金：广东省高等学校人才引进专项资金项目(粤财教[2013]246号);广东省自然科学基金项目(编号:S2011010002984)

摘　　要：目的观察高脂饲料对长爪沙鼠血清脂质4项和血清电解质K+、Na+、Cl-、Ca2+、Mg2+含量的影响。方法选取雄性长爪沙鼠40只,随机分为两组,分别给予普通饲料和高脂饲料(含1%胆固醇、10%猪油)。喂养12周后颌下静脉丛采血,用日立7180型全自动生化分析仪检测血清TC、TG、HDL-C、LDL-C及Ca2+、Mg2+含量,用美国Easy Lyte Plus型电解质分析仪检测血清K+、Na+、Cl-含量。结果与正常饲料组相比,高脂饲料喂养后沙鼠血清TC、TG、HDL-C、LDL-C含量显著升高(P<0.01),以TC、LDL-C水平升高最为明显。沙鼠血清电解质K+、Mg2+含量在不同饲料组差异有显著性(P<0.01或P<0.05),而Na+、Cl-、Ca2+含量在不同饲料喂养组的差异不显著(P>0.05)。结论高脂饲料能明显升高长爪沙鼠血清脂质4项,并改变其血清电解质K+、Mg2+水平。Objective To observe the effect of a high-fat diet on serum lipid parameters and electrolyte values of K^＋,Na^＋,Cl^-,Ca^2＋,Mg^2＋ in Mongolian gerbils. Methods Forty male Mongolian gerbils were selected and divided randomly into two groups（ 20 each group）. The first group of Mongolian gerbils were fed with a normal animal feed,while the second group were given a high-fat diet enriched with 1% cholesterol and 10% lard for twelve weeks. The serum samples were collected and tested for total cholesterol（ TC）,triglycerides（ TG）,high density lipoprotein cholesterol（ HDL-C）,low density lipoprotein cholesterol（ LDL-C）,and Ca^2＋,Mg^2＋ values using Hitachi 7180 auto-analyser,and for serum values of K^＋、Na^＋、Cl^- using Easy Lyte Plus electrolyte analyzer. Results Compared with normal animal feed group,the dietary intake of enriched with 1% cholesterol and 10% lard significantly increased the level of TC,TG,HLDL-C and LDL-C in the serum of Mongolian gerbils（ P〈0. 01）,and the serum level of TC and LDL-C were elevated remarkably. Meanwhile,the serum electrolyte K＋and Mg^2＋ values were also significantly increased（ P〈0. 01 and P〈0. 05）,while there were no significant difference in electrolyte Na^＋、 Cl^-、 Ca^2＋ values between both gerbils（ P〈0. 05）.Conclusion High-fat diet could significantly promote serum lipid parameters,and improve serum electrolyte K^＋、Mg^2＋ values in Mongolian gerbils.

关 键 词：长爪沙鼠 血清 脂质 电解质 

分 类 号：Q95-3[生物学—动物学]