紫外分光光度法与药典法测定安息香中总香脂酸含量比较  

Comparison of Determination of Total Balsamic Acids from Benzoinum between Ultraviolet Spectrophotometry and Chinese Pharmacopoeia Method

作　　者：黄小燕[1] 夏厚林[1] 宁梓君[1] 唐建军[1] 胡攀[1] 李瑞煜[1] 文冰杰 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室,四川成都611137

出　　处：《成都中医药大学学报》2014年第4期1-3,共3页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(编号:2007CB512606)

摘　　要：目的：建立紫外分光光度法（UV）测定安息香中总香脂酸含量,并比较紫外分光光度法与药典法（重量法）的差异,以探讨适宜的安息香总香脂酸含量测定方法。方法：采用2种方法测定相同安息香样品,比较2种方法的结果差异。结果：紫外分光光度法检测波长为223 nm,对照品苯甲酸在0.002~0.01 mg/m L线性关系良好,平均回收率为99.75%,RSD为2.75%。UV与重量法测得,测得同一批次（n=6）安息香总香脂酸平均含量为30.14%和31.48%,两种测定方法对测定结果相近,无显著性差异。结论：紫外分光光度法较药典法测定总香脂酸含量更为简便灵敏、结果准确可靠,可用于安息香总香脂酸的含量测定。Objective： To establish the method of ultraviolet spectrophotometry （UV） for the determination of total balsamic acids from Benzoinum and compare the difference of content between UV and Chinese Pharmacopoeia method （ gravimetric method） to investigate the suitable method for determination of total balsamic from Benzoinum. Methods： Total balsamic acis of the same Benzoi- num sample were determined by UV and gravimetric method, the results of two methods were compared. Results： The detecter wave- length of UV was set at 223nm. As the chemical reference, benzoic acid had a good linear relationship when the content of was within the range 0. 004 - 0. 01mg/mL. The average recovery of UV was 99. 75% , and RSD was 2. 75%. The average content of total balsamic acids in Benzoinum from the same batch determined by UV and gravimetric method was 30. 14% and 31.48%. There was no significant difference between the two methods for the content of total balsamic acids in Benzoinum. Conclusion ： Compared to Chinese pharmaco- poeia method, UV is sensitive and simple, and the determination result is accurate and reliable. It is suitable for the determination of total balsamic acids.

关 键 词：安息香 总香脂酸 紫外分光光度法 重量法 含量测定 

分 类 号：D284.1[政治法律—政治学] R282.71[政治法律—中共党史]