星点设计-效应面法优选罗布麻叶总黄酮提取工艺  

Optimization of Extraction Technology of Total Flavone in Apocynum venetum by Central Composite Design-Response Surface Methodology

作　　者：冉茂莲[1] 李小芳[1] 罗丽佳[1] 余琳[1] 罗丹[1] 李平[1] 李培培[1] 

机构地区：[1]成都中医药大学中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《成都中医药大学学报》2014年第4期4-7,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：四川省教育厅项目(编号:13ZB0315)

摘　　要：目的:采用星点设计-效应面法优化罗布麻总黄酮的提取工艺。方法:在预实验及单因素试验基础上,采用乙醇回流提取,并以乙醇体积分数、提取时间、料液比为自变量,罗布麻总黄酮得率为因变量,利用星点设计模型进行多元线性回归和二项式拟合,确定罗布麻的最佳提取工艺。结果:罗布麻的最佳提取工艺为23倍量65%乙醇,提取2次,150 min/次。得率实测值与预测值偏差为-1.60%,二项式拟合r=0.9870。结论:星点设计-效应面法优化罗布麻总黄酮提取工艺,方法简便,合理可行,预测性良好。Objective： To optimize extraction process of total flavone from Apocynum venetum by central composite design-re- sponse surface methodology. Methods： Based on preliminary experiments and single-factor tests, using ethanol reflux extraction, with the concentration of ethanol, extraction time, solid-liquid ratio as independent variables and yield of total flavone from Apocynum vene- tum as dependent variable, muhiple linear regression and binomial fitting was adopted by central composite design model, and deter- mine optimum extraction technology. Results： The best extraction process was as following： the amount of 65% ethanol, ratio of solid to liquid 1： 23, extraction times 2, reflux extraction time 150 min. Deviation of the predictive value and the measured value was - 1.60%. the regression coefficients of binomial fitting complex model was 0. 9870. Conclusion ： This optimized extraction process was simple and reliable, it was convenient and reasonable with high predictive.

关 键 词：罗布麻 总黄酮 星点设计 效应面法 

分 类 号：D284.2[政治法律—政治学] E282.71[政治法律—中共党史]