人卵巢颗粒细胞FoxO基因和卵泡刺激素受体基因的表达及相关性研究  

EXPRESSION AND RELATIONSHIP OF FoxO GENE AND FOLLICLE STIMULATING HORMONE RECEPTOR GENE IN HUMAN OVARIAN GRANULOSA CELLS

作　　者：梁莹[1] 米梅艳[2] 李淑贤[2] 周莉[1] 吴晓茜[1] 雷灵梅[3] 

机构地区：[1]河北省石家庄市第四医院生殖中心,河北石家庄050011 [2]河北省石家庄市第四医院妇科,河北石家庄050011 [3]河北省石家庄市第四医院检验科,河北石家庄050011

出　　处：《河北医科大学学报》2014年第10期1140-1143,共4页Journal of Hebei Medical University

基　　金：石家庄市科学技术研究与发展计划项目(11146753)

摘　　要：目的研究人卵巢颗粒细胞叉头框(forkhead box,Fox)基因和卵泡刺激素受体(follicle stimulating hormone receptor,FSHR)的表达及相关性。方法接受体外受精—胚胎移植(in vitro fertilization-embryo transfer,IVF-ET)不孕症患者126例,按照妊娠结果分为妊娠组63例,未妊娠组63例。回顾性分析取卵日成熟卵泡壁颗粒细胞FoxO1、FoxO3a和FSHR mRNA的表达,并对FoxO1、FoxO3a、FSHR之间及与血清促黄体生成素(1uteotropic hormone,LH)、雌二醇(estradiol,E_2)、孕酮(progesterone,P)进行相关性分析。结果妊娠组血清E_2水平高于未妊娠组(P<0.01);妊娠组优质胚胎数多于未妊娠组(P<0.01);2组LH、P、获卵数差异无统计学意义(P>0.05)。妊娠组FoxO1 mRNA表达高于未妊娠组(P<0.01);2组FoxO3a及FSHR mRNA差异无统计学意义(P>0.05)。FoxO1和FoxO3a与FSHR的相关性系数分别为0.881、0.999(P<0.01i)。结论 FoxO1和FoxO3a参与卵母细胞质量的调节,其功能与FSHR有密切关系。Objective To explore the expression of forkhead box（FoxO）gene and follicle-stimulating hormone receptor （FSHR）in human ovarian granulosa cells and their relationship. Methods One hundred and twenty-six unfertility women treated with in vitro fertilization and embryo transplantation （IVF-ET ） were recruited.According to the pregnancy outcome,the patients were divided into pregnancy groups 63 cases and non-pregnancy group 63 cases.The expressions of FoxO1 mRNA,FoxO3a mRNA and FSHR mRNA on human ovarian granulosa cells were detected by real time fluorescence quantitative PCR.The correlation analysis of FoxO1, FoxO3a,FSHR with serum sex hormones including luteotropic hormone（LH）,estradiol（E2 ）, progesterone（P）were mevaluated.Results High quality embryo number and serum E2 level in pregnancy group were significantly higher than those of non-pregnancy group （P〈0.01 ）,but＆amp;nbsp;LH,P level and number of eggs showed no significant difference between two groups （P〉0.05）. The expression FoxO1 mRNA in pregnancy group was higher than that of non-pregnancy group （P〈0.01）.The expression of FoxO3a and FSHR mRNA were no statistical difference between pregnancy group and non-pregnancy group （P〉0.05）.The correlation coefficients of FoxO1 and FoxO3a with FSHR were 0.881,0.999 （P〈0.01 ）.Conclusion FoxO1 and FoxO3a genes participate in the regulation of oocyte quality and their functions have closely connected with FSHR.

关 键 词：卵泡刺激素 人 卵丘细胞 卵母细胞 

分 类 号：R715.9[医药卫生—妇产科学]