大鼠发热过程中脑腹中膈区TRPV1及AVPV1受体的表达  

Expression of TRPV1 and AVPV1 Receptors in the Ventral Septal Area of Rat during Fever

作　　者：曹志友[1] 施蓓[1] 单悦梅[1] 张璠[1] 秦鑫[1] 曹宇[1] 

机构地区：[1]中国医科大学基础医学院生理学教研室,沈阳110001

出　　处：《中国医科大学学报》2014年第11期965-968,972,共5页Journal of China Medical University

基　　金：国家自然科学基金主任基金(31340074);辽宁省自然科学基金(201202277)

摘　　要：目的 观察在脂多糖致大鼠发热过程中脑腹中膈区（VSA）瞬时受体电位香草酸受体1（TRPV1）及精氨酸加压素（AVP）V1受体表达水平的变化.方法 实验动物随机分为4组：正常对照（N）组、Capsazepine（C）组、脂多糖（L）组和Capsazepine＋脂多糖（C＋L）组.连续观察体温变化;对不同实验条件下VSA中TRPV1的表达采用Western blot方法检测,AVPV1受体mRNA水平变化采用RT-PCR方法检测.结果 L组和C＋L组体温显著增高,C＋L组210～510 min期间体温明显高于L组（P＜0.01）.TRPV1的表达水平显示致热后逐渐增多,L组最高值为基础状态的2.2倍.C＋L组为1.72倍.同样,AVPV1受体mRNA表达水平出现相似的变化趋势,L组AVPV1受体mRNA表达最大量为基础状态的2.4倍.C＋L组为1.95倍.结论 LPS致大鼠发热时,体温升高到一定程度可诱导腹中膈区TRPV1的表达,并伴有AVPV1受体mRNA表达水平升高,此变化有利于AVP发挥对体温的负调节作用.Objective To evaluate of expression levels of transient receptor potential vanilloid receptor 1 （TRPV1） and arginine vasopressin （AVP） V1 receptors in the ventral septal area（VSA） of rat during fever.Methods The male rats were randomly divided into four groups：the control group （N）,the Capsazepine group （CPZ）,the LPS group （L） and the Capsazepine＋LPS group （CPZ＋LPS）.Body temperature of the rats were continuously observed.TRPV1 expression was detected by Western blot and AVPV1 mRNA lever was determined by RT-PCR in the VSA.Results The body temperature of the rats were increased in both L group and C＋L group; the body temperature was significantly higher in C＋L group than that in L group during 210-510 min （P＜ 0.01）.The level of TRPV1 expression was gradually increased after the administration of LPS.The highest value in the L group was 2.2 times than that of the basal state,and in the C＋L group the value was 1.72 times.Meanwhile,the mRNA level of AVPV1 appeared the similar trend.The maximum expression of A VPV1 mRNA level in group L was 2.4 times than that of the basal state,and the value was 1.95 times in the C＋L group.Conclusion During the LPS-induced fever,whenever the temperature was rose to a certain degree,the TRPV1 expression could be induced in the VSA and the AVPV1 mRNA level could also be increased,which may play a negative effect on the regulation of the body temperature.

关 键 词：发热 瞬时受体电位香草酸受体1 精氨酸加压素V1受体 腹中膈 

分 类 号：R332[医药卫生—人体生理学]