石斛合剂序贯法治疗糖尿病大鼠的基因和蛋白表达谱之糖代谢研究  

Gene and Protein Expression Spectrum Research of Clinical Sequential Treatment of Dendrobium Compound(DC) in Diabetic Rats about Glucose Metabolism

作　　者：林心君[1] 许茜[1] 刘赟[1] 崔乙[2] 余文珍[1] 王晓宁[1] 施红[1] 

机构地区：[1]福建中医药大学中西医结合学院,福建福州350122 [2]福建医科大学医学技术与工程学院,福建福州350004

出　　处：《中华中医药学刊》2014年第12期2857-2860,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81072736)

摘　　要：目的:采用基因和蛋白表达谱芯片研究,观察石斛合剂序贯疗法对糖尿病大鼠糖代谢的影响。方法:选取健康雌性Wistar大鼠50只,随机分为正常组(10只)和实验组(40只),正常组给予基础饲料喂养,实验组给予高脂高糖饲料喂养16个月后腹腔注射STZ造模。将成模大鼠随机分为模型组、二甲双胍组、石斛合剂序贯方组(以下简称序贯组)。相应药物连续灌胃16周后,检测大鼠FBG、INS、GLU、GSP,并行肝组织基因及蛋白芯片检测。结果:1与模型组比,序贯组FBG、GSP、GLU水平均显著下降(P<0.01)。2模型组与正常组存在差异的已知功能基因1339个(P<0.05),经石斛合剂序贯方治疗后仅存差异380个(P<0.05)。3蛋白芯片结果显示:序贯组INS、IR、IRS-1、IGF-1、PPAR-γ、Glicentin水平均较模型组升高。结论:石斛合剂序贯疗法有良好的降糖效果和综合疗效,其降糖机制可能与调节多条糖代谢通路组分的表达有关。Objective： To observe the effects of Dendrobium compound sequential therapy on diabetic glucose metabolism by the research of gene and protein expression spectrum. Methods： 50 female Wistar rats of SPF degree,weighed（ 250± 20） g,were randomly divided into 2 groups： the normal control group（ 10） and the experimental group（ 40）. The normal control group was fed with basic diet. The experimental group was fed with high- fat high- sugar diet for 16 months and followed by 2 intraperitoneal（ STZ） injections to establish models. Successful modeling rats were randomly divided into model group,Dendrobium Compound（ DC） sequential group and metformin group. Sixteen weeks continuous gavage,we detected rats＇ fasting blood- glucose（ FBG）,insulin（ INS）,glucagon（ GLU） and glucosylated serum protein（ GSP）.The liver of the rats in three groups（ control,model and DC） was used for Agilent genome expression microarray testing and protein- chip testing. Results： 1Compared with model group,the levels of FBG,INS and GLU were significantly lower（ P〈0. 01）,but the level of INS showed no difference（ P〈0. 05）. 2Between the model group and the normal control group,1339 functional genes showed differences in expression levels（ P〈0. 05）. After DC sequential treatment,the expression level of nearly 1000 genes returned to normal（ compared with the normal control group,P〈0. 05）,only 380 genes showed differences in expression（ P〈0. 05）. 3 The result of the protein- chip testing showed： Compared with model group,the levels of Insulin,IR,IRS- 1,IGF- 1,PPAR- γ and Glicentin increased after DC sequential treatment.Conclusion： Dendrobium Compound sequential treatment could effectively reduce glucose and has a comprehensive effect on the model rats. Its mechanism was associated with regulating the expressions of components in multi- glucose metabolism pathways.

关 键 词：石斛合剂序贯法 糖尿病 糖代谢 基因芯片 蛋白芯片 

分 类 号：R285.5[医药卫生—中药学]