绞股蓝总皂苷对光老化HSF细胞MMP-3、TIMP-2mRNA及蛋白表达影响的实验研究  

Experimental Study of Gypenosides-Containing Serum on Human Skin Fibroblast Cell Line(HSF) MMP-3 and TIMP-2 mRNA and Protein Expression of Photoaging HSF Cells

作　　者：张小卿[1] 马贤德[1] 田国伟[1] 张志星[1] 张燚[2] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属第三医院,辽宁沈阳110003

出　　处：《中华中医药学刊》2014年第12期2944-2947,I0038,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省自然科学基金项目(201102150)

摘　　要：目的:观察绞股蓝总皂苷(gypenosides,GPs)含药血清对光老化人皮肤成纤维细胞株(HSF)MMP-3和TIMP-2 mRNA及蛋白表达的影响。方法:采用长波紫外线(UVA)照射方法建立老化HSF细胞模型,并给予含不同浓度GPs大鼠血清进行干预,采用逆转录-聚合酶链反应(RT-PCR)和蛋白印迹(Western-blot)法,分别对各组细胞MMP-3与TIMP-2 mRNA和蛋白表达进行检测。结果:与空白对照组比较,UV模型组细胞内MMP-3基因和蛋白表达显著增加,同时TIMP-2基因和蛋白表达显著减少。与UV模型组比较,低、中、高剂量含药血清组细胞内MMP-3基因和蛋白表达显著下降,TIMP-2基因和蛋白表达显著上升。结论:GPs通过抑制HSF细胞MMP-3表达,同时促进TIMP-2表达,恢复MMP-3与TIMP-2表达平衡状态,抑制基质金属蛋白酶对细胞外基质(胶原蛋白、蛋白多糖)的降解,为GPs抗皮肤光老化作用机制之一。Objective： To study the effect of gypenosides- containing serum on human skin fibroblast cell line（ HSF）MMP- 3 and TIMP- 2 mRNA and protein expression of skin photoaging cells. Method： Long- wave ultraviolet（ UVA）irradiation method was adopted to establish the HSF cell aging model. After the models were interfered with rat serum containing different concentrations of Gypenosides,MMP- 3 and TIMP- 2 mRNA and protein expression were detected with reverse transcription- polymerase chain reaction（ RT- PCR） and protein imprinting method（ Western blot） respectively. Results： Compared with blank control group,MMP- 3 gene and protein expression in cells of UV model group increased significantly,at the same time TIMP- 2 gene and protein expression significantly reduced. Compared with UV model group,MMP- 3 gene and protein expression in cells of gypenosides- containing serum group decreased significantly,TIMP- 2 gene and protein expression increased significantly. Conclusion： GPs can inhibit the expression of MMP- 3and promote the expression of TIMP- 2 at the same time and restore MMP- 3 and TIMP- 2 expression of equilibrium and inhibit MMPS of extracellular matrix（ collagen and proteoglycan） degradation. This might be one of the mechanisms of anti photoaging of GPs.

关 键 词：绞股蓝总皂苷 光老化 皮肤成纤维细胞 基质金属蛋白酶 金属蛋白酶组织抑制剂 

分 类 号：R285.5[医药卫生—中药学]