电针内关穴对体外循环大鼠心肌细胞凋亡和Bax、Bcl-2蛋白表达的影响  

Effects of electroacupuncture at Neiguan point on myocardial apoptosis and the expression of apoptosis- related protein Bax and Bcl- 2 in rats undergoing cardiopulmonary bypass

作　　者：涂杰[1] 梁蓓薇[1] 梁东科[1] 韦秋英[1] 李涛[1] 张炳东[1] 

机构地区：[1]广西医科大学第一附属医院心血管病研究所手术麻醉室,南宁530021

出　　处：《广东医学》2014年第21期3299-3301,共3页Guangdong Medical Journal

基　　金：广西自然科学基金资助项目(编号:2013GXNSFBA019124)

摘　　要：目的评价电针内关穴对体外循环（CPB）大鼠心肌细胞凋亡与相关蛋白Bax、Bcl-2表达的影响。方法成年雄性SD大鼠60只,6~8月龄,体重400~450 g。采用随机数字表法,将大鼠随机分为3组（n=20）：假手术组（S组）、CPB组和电针内关穴组（EA组）。采用尾动脉插管灌注,右颈静脉插管引流建立CPB模型。S组行麻醉和穿刺但不行CPB;CPB组行CPB 1 h;EA组行CPB 1h且在CPB期间使用电针刺激双侧内关穴。CPB结束后2 h处死大鼠并取左心室心肌组织,TUNEL法检测心肌细胞凋亡情况,Western blot法检测Bax和Bcl-2蛋白表达水平。结果与S组比较,CPB组和EA组心肌细胞凋亡指数升高,Bax和Bcl-2蛋白表达上调,Bax/Bcl-2比值上升（P〈0.05）;与CPB组比较,EA组心肌细胞凋亡指数下降,Bax蛋白表达下调,Bcl-2蛋白表达上调,Bax/Bcl-2比值下降（P〈0.05）。结论电针内关穴对CPB大鼠的心肌细胞有明显的保护作用,其机制可能与调节凋亡相关蛋白Bax和Bcl-2的表达从而抑制细胞凋亡有关。Objective To investigate the effects of electroacupuncture at Neiguan point on myocardial apoptosis and the expression of apoptosis - relaled protein Bax and Bcl - 2 in rats undergoing cardiopulmonary bypass （CPB）. Methods Sixty adult male Sprague - Dawley rats, aged 6 - 8 months, weighing 400 --450 g, were randomly divided into 3 groups （n = 20 each） ： Sham operation group （Group S） , Group CPB and electroacupuncture at Neiguan point group （ Group EA）. Tail arteries and right jugular vein were eannulated for CPB. Rats in Group S were anaesthetized and eanuu- lated without CPB; rats in Group CPB were subjected to CPB for 1 h; and rats in Group EA were subjected to CPB for 1 h and eleetroacupuncture at Neiguan point during CPB. Those animals were sacrificed 2 hours after CPB. Myocardium specimens were obtained from left ventricle for determination of apoptosis by TUNEL method, and the protein expression of Bax and Bcl -2 by Western blot. Results Compared with Group S, the apoptosis index of myocardial cells and ratio of Bax/Bcl - 2 were significantly increased in Group CPB and Group EA （ P 〈 0. 05 ） , with significant up - regulation of the expression of Bax and Bel - 2 （ P 〈 0. 05 ）. Compared with Group CPB, the apoptosis index of myocardial cells and ratio of Bax/Bel - 2 were significantly reduced in Group EA （ P 〈 0. 05 ）, with significant down - regulation of Bax and up - regu- lation of Bcl - 2 （ P 〈 0. 05 ）. Conclusion Eleetroacupuncture at Neiguan has protective effects on myocardial cells in rats undergoing CPB, the mechanism is closely related to inhibiting myocardial apoptosis by adjusting the expression of apopto- sis -related protein Bax and Bcl- 2.

关 键 词：电针 内关穴 体外循环 心肌细胞 凋亡 BAX Bcl-2 

分 类 号：R977.11[医药卫生—药品]