Development of a multi-component drug from turmeric using central composite design  

作　　者：Xiaohang ZHANG Shengnan HAN Yan LI Jianlan JIANG 

机构地区：[1]Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

出　　处：《Frontiers of Chemical Science and Engineering》2014年第3期362-368,共7页化学科学与工程前沿（英文版）

摘　　要：A methodology drugs based on traditional to develop multi-component Chinese medicines has been developed using central composite design. Several active components from the traditional Chinese medicine turmeric were chosen for use in a multi-component antitumor drug. Response surface methodology based on a central composite design was applied to determine the quantitative composition-activity relationships in order to optimize the amount of each component in the drug. An MTT （3-（4,5- dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide） assay was used to measure the pharmacological activity as the response value. The experimental antitumor activity of the optimum combination was 92.85% in the MTT assay and superior to the activities of each single component. These results demonstrate that response surface methodology based on a central composite design is suitable for the design of multi-component drugs.A methodology drugs based on traditional to develop multi-component Chinese medicines has been developed using central composite design. Several active components from the traditional Chinese medicine turmeric were chosen for use in a multi-component antitumor drug. Response surface methodology based on a central composite design was applied to determine the quantitative composition-activity relationships in order to optimize the amount of each component in the drug. An MTT （3-（4,5- dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide） assay was used to measure the pharmacological activity as the response value. The experimental antitumor activity of the optimum combination was 92.85% in the MTT assay and superior to the activities of each single component. These results demonstrate that response surface methodology based on a central composite design is suitable for the design of multi-component drugs.

关 键 词：multi-component drug development centralcomposite design turmeric CURCUMINOIDS 

分 类 号：TQ92[轻工技术与工程—发酵工程] O657.3[理学—分析化学]