曙红Y化学发光体系的建立及其用于妥布霉素滴眼液的测定  

Establishment of eosin Y chemiluminescence system and its application in the determination of tobramycin in eye drops

作　　者：刘贞[1] 王瑞[1] 崔晓然[1] 陈建秋[1] 严拯宇[1] 

机构地区：[1]中国药科大学分析化学教研室,南京210009

出　　处：《中南药学》2014年第10期965-969,共5页Central South Pharmacy

基　　金：大学生创新药物研制能力提高项目(No.J1030830);国家级大学生创新创业训练计划项目(No.G13003)

摘　　要：目的建立曙红Y（EY）化学发光体系快速测定妥布霉素滴眼液的方法。方法在强碱性条件下EY与N-溴代丁二酰亚胺（NBS）发生作用,产生较强的化学发光（CL）,妥布霉素对该体系的发光信号有增敏作用。当NBS为0.889 9 mg·mL^-1、NaOH为20 mg·mL^-1、EY为0.484 3 mg·mL^-1时,用于妥布霉素的测定。结果妥布霉素在0.101 8~10.18μg·mL^-1内线性关系良好,线性相关系数r为0.999 6,检出限为6.388×10^-2μg·mL^-1。结论本方法分析速度快、灵敏度高,可以用于妥布霉素滴眼液的快速分析检测。Objective To establish a chemilumin escence（CL） meth od to determine t obramycin in eye drops. Metho ds A clear CL signal was recorded when eosin Y（EY） dissolved in the strong alkaline solution was mixed with N-bromosuccinimide（NBS）. The CL signal was enhanced by the addition of tobramycin into the EY-NBS system. Tobramycin was determined when the concentrations of NBS, NaOH and EY were 0.889 9 mg·mL^- 1, 20 mg·mL^- 1 and 0.484 3 mg·mL^- 1 respectively. Results T he increase in the CL intensity had a good linearity with the tobramycin concentration. Good linearity was obtained with a coeffi cie nt of 0.999 6 at 0.101 8- 10.18 μg·mL-1 and a detection limit of 6.388×10-2 μg·mL^-1. Conclusion This study provides a simple and quick method to detect to bramycin in eye drops.

关 键 词：曙红Y N-溴代丁二酰亚胺 妥布霉素 化学发光 

分 类 号：R917[医药卫生—药物分析学]