高效液相色谱法测定甘草远志合剂中4种指标成分的含量  

HPLC determination of 4 components in Gancao Yuanzhi mixture

作　　者：庄珊珊[1,2] 刘志宏 黄爱文 宋洪涛 

机构地区：[1]南京军区福州总院药学科,福州350025 [2]福建医科大学药学院,福州350108

出　　处：《中南药学》2014年第10期1005-1008,共4页Central South Pharmacy

基　　金：军队医疗机构制剂标准提高科研专项课题(编号:13ZJZ01)

摘　　要：目的建立高效液相色谱法同时测定甘草远志合剂中甘草苷、甘草酸铵、3,6＇-二芥子酰基蔗糖和细叶远志皂苷的含量。方法采用Kromasil C18色谱柱（4.6 mm×150 mm,5μm）,以乙腈为流动相A,以0.1%磷酸溶液为流动相B进行梯度洗脱,流速1.0 mL·min-1,检测波长分别为210、237 nm,柱温30℃。结果甘草苷在10.0~150.0μg·mL^-1（r=0.999 9）,甘草酸铵在50~1 000μg·mL^-1（r=0.999 8）,3,6＇-二芥子酰基蔗糖在2~50μg·mL^-1（r=0.999 7）和细叶远志皂苷在5.0~130.0μg·mL^-1（r=0.999 9）与峰面积呈良好的线性关系,低、中、高3个浓度的平均加样回收率（n=3）均〉97%,RSD均〈3%,溶液在24 h内稳定。结论本法快速简便、准确可靠、重复性好,可用于测定甘草远志合剂中甘草苷、甘草酸铵、3,6＇-二芥子酰基蔗糖和细叶远志皂苷的含量,为甘草远志合剂及其类似制剂的质量评价提供依据。Objective To establish an HPLC method to determine the content of liquiritin, ammonium glycyrrhizate, 3, 6＇-disinapoylsucrose and tenuifolin in Gancao Yuanzhi mixture. Methods The Kromasil C18（4.6 mm×150 mm, 5 μm） column was used with a mobile phase of acetonitrile（A）-0.1% phosphoric acid（B）. The flow rate was 1.0 mL·min^- 1, the detection wavelength were 210 nm and 237 nm, and the column temperature was 30 ℃. Results The liquiritin, ammonium glycyrrhizate, 3, 6＇-disinapoylsucrose and tenuifolin had a good linearity at 10.0- 150.0 μg·mL-1（ r = 0.999 9）, 50- 1 000 μg·mL^-1（r = 0.999 8）, 2- 50 μg·mL-1（r = 0.999 7）, and 5.0- 130.0 μg·mL^-1（r = 0.999 9）. The average recoveries were 97%（n = 3）, the RSDs were less than 3%, stablely within 24 h. Conclusion The method is convenient, rapid, accurate and reliable, which can be used for the determination of liquiritin, ammonium glycyrrhizate, 3, 6＇-disinapoylsucrose and tenuifolin in Gancao Yuanzhi mixture. This study provides scientifi c basis for the quality evaluation of Gancao Yuanzhi mixture and preparations of the same kind.

关 键 词：甘草远志合剂 高效液相色谱法 甘草苷 甘草酸铵 3 6'-二芥子酰基蔗糖 细叶远志皂苷 

分 类 号：R917[医药卫生—药物分析学]