SBR和MBR反应器中好氧反硝化菌的筛选与分析  

Selection and Analysis of Aerobic Denitrification Strain from SBR and MBR Reactors

作　　者：李安峰[1,2] 骆坚平[1,2] 刘玉娟[1,2] 潘涛[1,2] 

机构地区：[1]北京市环境保护科学研究院,北京100037 [2]国家城市环境污染控制工程技术研究中心,北京100037

出　　处：《微生物学杂志》2014年第5期65-69,共5页Journal of Microbiology

基　　金：北京市环境保护科学研究院科技基金(2013-A-07)

摘　　要：好氧反硝化菌是土壤、沉积物、活性污泥等介质中广泛存在的一类微生物。研究以相同种泥扩大培养的SBR(序批式活性污泥反应器)和MBR(膜生物反应器)中活性污泥为研究对象,采用平板分离法分别从运行6个月的SBR和MBR反应器中分离得到9株和11株好氧反硝化菌。经好氧反硝化性能测定发现,20株细菌中有16株总氮去除率在50%以上,且NO_2^--N累积量均在0.5 mg/L以下。经系统进化分析表明,在门分类级别中,采用同一种泥的2个反应器中好氧反硝化菌表现出极大的一致性,20株好氧反硝化菌全部属于变形菌门(Proteobacteria)。但是,进一步分析发现不同反应器中好氧反硝化菌表现出较大的属、种间的差异。其中,SBR反应器9株好氧反硝化菌分属于4个属,6个物种;而从MBR反应器获得的11株细菌分属于2个属,6个物种;仅1个物种(Pseudomonas toyotomiensis)在2个反应器均有发现。Aerobic denitrification strains （ADS） exist widely in media such as soil,sediment and activated sludge.In this study,activated sludge from a sequencing batch reactor （SBR） and a membrane biological reactor （MBR） with the same seed sludge were studied.9 and 1 1 ADS were isolated from SBR and MBR respectively adopting plate isolation method,after an acclimation period for 6 months.Among them 20 strains of ADS,16 strains could effectively remove total nitrogen with a removal rate of more than 50％ and nitrite nitrogen with a concentration of lower than 0.5 mg/L.Phylogenetic analyses demonstrated that all ADS from the two reactors belong to Proteobacteria with great consistency in the taxonomy of phylum,but they were significantly different in the taxonomy of genus and species.The 9 strains from SBR were distributed in 4 genera and 6 species while the 11 strains from MBR in 2 genera and 9 species.Only one species （Pseudomonas toyotomiensis） was found in both of the reactors.

关 键 词：好氧反硝化菌 筛选 SBR MBR 活性污泥 

分 类 号：Q933[生物学—微生物学]