复健片对局灶性脑梗死大鼠颈髓ROCK2的影响  

Effects of Fujian Tablet on ROCK2 expression of cervical spinal cord in focal cerebral infarction rats

作　　者：刘伟[1] 张国丽[2] 

机构地区：[1]山东中医药大学第一临床医学院,济南250011 [2]山东省肿瘤防治研究院,济南250117

出　　处：《中华中医药杂志》2014年第11期3436-3439,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81273701)~~

摘　　要：目的:探讨滋补肝肾复方复健片对局灶性脑梗死大鼠颈髓Rho相关卷曲螺旋形成蛋白激酶2(ROCK2)的作用。方法:采用右侧近端大脑中动脉电凝术制备大脑中动脉阻塞(MCAO)模型。动物随机分为假手术组、模型组、药物组,其中3组又各分为术后4周组和术后5周组,每组各8只。药物组按9g生药/kg给予复健片水溶液,其余两组分别给予同等量0.9%氯化钠溶液,1次/d。分别采用RT-PCR和Western blot方法检测大鼠左侧颈髓ROCK2 mRNA和蛋白的表达。结果:术后4周末,模型组与药物组的ROCK2 mRNA和蛋白表达均较假手术组显著性降低(P<0.05,P<0.01),而药物组较模型组降低更为显著(P<0.01),至术后5周末;模型组与假手术组ROCK2 mRNA和蛋白表达已无显著差异,但药物组仍然显著低于其余两组(P<0.01,P<0.05)。结论:复健片可抑制MCAO大鼠失神经支配侧颈髓ROCK2基因和蛋白的表达,从而有助于促进局灶性脑梗死皮质脊髓束重塑。Objective： To investigate the effects of Fujian Tablet, a compound nourishing the liver and the kidney, c n ROCK2 expression of cervical spinal cord in focal cerebral infarction rat. Methods： Experimental rats were randomly divided into： sham operation group, model group and Fujian Tablet group （FJT group）. The right middle cerebral artery occlusions （MCAO） rat model was established by the electrocoagulation. Each group was randomly divided into 2 subgroups： 4 weeks after operation subgroups and 5 weeks after operation subgroups, with 8 rats in each subgroup. FJT group were intragastric administration with Fujian Tablet decoction at 9g crude drug per kg of rat body weight once a day, while the other groups were given equal dosage of normal saline. RT-PCR was used to detect the expression of ROCK2 mRNA and Western-blot was used to measure the expression of ROCK2 protein in the left cervical spinal cord of experimental rat. Results： At the end of 4 weeks after operation, the expressions of ROCK2 mRNA and protein in model group and FJT group were both significantly lower than those in the shara operation group （P〈0.05, P〈0.01）, while the expressions of ROCK2 mRNA and protein in FJT group were both significantly lower than those in the model group （P〈0.01）. At the end of 5 weeks after operation, there was no difference in the expressions of ROCK2 mRNA and protein between the model group and the sham operation group, but the expressions of ROCK2 mRNA and protein in FJT group were both lower than those in the other groups （P〈0.01, P〈0.05）. Conclusions： Fujian Tablet can inhibit th,~ expression of Rock2 mRNA and protein in cervical spinal cord of MCAO rats, which will facilitate corticospinal tract plasticity of focal cerebral infarction.

关 键 词：局灶性脑梗死 颈髓 Rho相关卷曲螺旋形成蛋白激酶2 复健片 

分 类 号：R285.5[医药卫生—中药学]