虚寒证、虚热证线粒体呼吸链复合体Ⅰ活性的研究  

Research on respiratory chain complex I activity of mitochondrion on deficiency-cold syndrome and deficiency-heat syndrome

作　　者：杨丽萍[1] 闫盼盼[1] 黄睿[1] 李新民[1] 侯俊林[1] 高爱社[1] 詹向红[1] 

机构地区：[1]河南中医学院中西医结合基础学科,郑州450046

出　　处：《中华中医药杂志》2014年第11期3440-3442,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81072734);河南省杰出青年基金(No.114100510011);河南省教育厅自然科学项目(No.2010A360027);河南中医学院创新人才项目(No.2010XCXRC01);河南中医学院研究生创新基金项目(No.2012YCX009)~~

摘　　要：目的:通过研究虚寒证、虚热证人群在不同环境温度状态下线粒体呼吸链复合体Ⅰ(COⅠ)活性的变化,探讨COⅠ活性在虚寒证、虚热证人群中的变化规律。方法:通过量表和专家诊断筛选出虚寒证组、虚热证组及正常对照组各20名,采集两种温度下血液,分光光度法测定COⅠ活性值。结果:两种环境温度下,与正常对照组相比,虚寒证组COⅠ活性值均显著降低(P<0.05);虚寒证组与虚热证组COⅠ活性值差异均有统计学意义(P<0.05);与室温相比,低温下3组COⅠ活性值均增大,且正常对照组两种温度下COⅠ活性值差别有统计学意义(P<0.05),COⅠ活性调节速率的差异有统计学意义(P<0.05)。结论:不同证型组COⅠ活性不同,表现为虚寒证组<正常对照组<虚热证组,虚寒证组、虚热证组COⅠ活性随环境温度变化且其调节能力均低于正常对照组。Objective： To study the change of respiratory chain complex I activity of mitochondrion in crowd with deficiency-cold syndrome and deficiency-heat syndrome under various ambient temperatures, and to investigate the change rule of complex I activity. Methods： The subjects were selected by questionnaire and expert diagnosis, and were divided into normal control group, deficiency-cold syndrome group and deficiency-heat syndrome group, 20 people in each group. Their blood samples were collected at two different temperature conditions. The complex I activity was detected by using spectrophotometry. Results： ①Compared with the normal control group at the two different temperatures, the value of complex I activity reduced in deficiency-cold syndrome group, the difference was statistically significant （P〈0.05）, the value of complex I activity was significantly different between deficiency-cold syndrome group and deficiency-heat syndrome group （P〈0.05）; ②Compared with room temperature, the value of complex I activity in three groups all raised at cold temperature, and the value and the regulation speed of complex I all had significant difference （P〈0.05） in normal control group under two different temperature. Conclusion： The complex I activity is different in various syndrome group, which all showed that deficiency-cold syndrome group〈normal control group 〈 deficiency-heat syndrome group. For the deficiency-cold syndrome group and deficiency-heat syndrome group, the ability of regulation with environment temperature change is lower than the normal control group.

关 键 词：虚寒证 虚热证 复合体I 能量代谢 

分 类 号：R259[医药卫生—中西医结合]