糖肾方对糖尿病肾病大鼠肾脏TLR4及MCP-1表达的影响  

Effects of Tangshen Formula on the expression of TLR4 and MCP-1 in rats with diabetic nephropathy

作　　者：吴芊葭[1] 胡冬和[1] 黄蔚霞[1] 董飞侠[1] 程锦国[1] 

机构地区：[1]温州市中医院,温州325000

出　　处：《中华中医药杂志》2014年第11期3610-3613,共4页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:探讨糖肾方对糖尿病肾病(DN)大鼠Toll样受体4(TLR4)及单核细胞趋化蛋白-1(MCP-1)表达的影响。方法:40只雄性SD大鼠单侧肾切除后分为空白组(10只)和实验组(30只)、实验组通过单次STZ腹腔注射诱导DN模型,再随机分为:模型组、中药组、氯沙坦组。以灌胃的方式给予各组分别干预8周,应用免疫组化法及实时荧光定量PCR法检测大鼠肾组织TLR4、MCP-1蛋白及m RNA表达情况。结果:模型组肾组织TLR4和MCP-1蛋白及m RNA表达水平明显高于空白组(P<0.05);中药组各指标均显著低于模型组(P<0.05),与氯沙坦组比较,无明显差异。结论:糖肾方能够下调糖尿病肾病大鼠肾组织TLR4和MCP-1的表达,从而减轻肾组织炎症反应,发挥其肾脏保护作用。Objective： To investigate the effect of Tangshen Formula on the expression of toll-like receptor 4（TLR4） and monocyte chemotactic protein I（MCP-1） in rats with diabetic nephropathy （DN）. Methods： After underwent unilateral nephrectomy, 40 male SD rats with were randomly divided into normal group （n=10） and experimental group （n=30）. Rat model of DN was established by intraperitoneal injection of streptozotocin （STZ） in the experimental group, and then DN rats were randomly divided into three groups： model group, TCM group and losartan group. Each group was respectively administered intragastrically with corresponding intervention for 8 weeks. Protein expression of TLR4 and MCP-1 was detected by immunohistochemistry method, and mRNA expression of TLR4 and MCP-1 was detected by RT-PCR. Results： Compared with the normal group, the protein and mRNA expression of TLR4 and MCP-1 were obviously higher in DN rats （P〈0.05）, Besides, the indexes in TCM group were lower than those in model group （P〈0.05）, and there was no significant difference compared with losartan group. Conclusion： Tangshen Formula had a certain protective effect on kidney in DN rats. It could reduce the inflammation through down-regulating the expression of TLR4 and MCP-1.

关 键 词：糖肾方 糖尿病肾病 TOLL样受体4 单核细胞趋势化蛋白-1 炎性反应 

分 类 号：R285.5[医药卫生—中药学]