芥子碱对小鼠肝癌H22的体内抑制作用初探  

Preliminary study on anti-tumor effect of sinapine on mice with H22 hepatocellular carcinoma

作　　者：南淑蕾[1] 吴国欣[2] 何珊[2] 黄鹭强[2] 张福华[2] 

机构地区：[1]三明学院,三明365004 [2]福建师范大学,福州350108

出　　处：《中华中医药杂志》2014年第11期3618-3622,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：福建省自然科学基金项目(No.C0410010);福建省科技厅科技项目基金(No.2008F5017)~~

摘　　要：目的:研究芥子碱对小鼠肝癌H22的体内抑制作用及其机制。方法:通过建立小鼠肝癌H22的腹水型和实体瘤模型来观察芥子碱的抑瘤作用,并采用HE染色法观察细胞形态。通过胸腺指数、脾脏指数分析芥子碱对小鼠免疫器官的影响,并采用丫啶橙染色观察细胞凋亡形态,琼脂糖凝胶电泳及免疫组化方法研究芥子碱的诱导细胞凋亡机制。结果:芥子碱大、中、小剂量组对小鼠肝癌H22实体瘤的抑制率分别为40.3%、28.4%、20.8%,但芥子碱对腹水型H22小鼠的生命延长率的影响则无显著提高;芥子碱各剂量组促凋亡因子Bax的表达量不同程度地增加(P<0.01)、抗凋亡因子Bcl-2的表达量不同程度地减少(P<0.01)。结论:芥子碱对小鼠肝癌H22实体瘤有显著的抑制作用,存在剂量依赖效应,且未出现明显毒副作用,而免疫增强和促细胞凋亡是芥子碱实现对小鼠肝癌H22实体瘤的抑制作用的可能途径。Objective： To study on the anti-tumor effect and mechanism of sinapine on mice with H22 hepatocellular carcinoma. Methods： The ascitic type and solid tumor of H22 were established to observe the anti-tumor effect of sinapine. HE staining was used to observe the cellular morphology. The thymus index and spleen index were used to analyze the influence of sinapine on the immune organs. Apoptosis morphology of cells was observed by using acridine orange （OA） stain. Agarose gel electrophoresis （AGE） and immunohistochemical were used to study the apoptosis mechanism induced by sinapine Results： The solid tumor inhibition ratio of high, middle and low dose of sinapine treated groups were 40.3%, 28.4% and 20.8% respectively, but there was no significant effect of the influence of sinapine on the life extension of H22 ascites type mice. Sinapine could promote the expression of Bax （P〈0.01） and control the expression of Bcl-2 （P〈0.01）. Conclusion： Sinapine could inhibit the H22 solid tumor on mice significantly with dose-dependent and no obvious side effect. The probable route of inhibitory effect of sinapine on mice with H22 solid tumor is immune-enhancement and acceleration of cell apoptosis.

关 键 词：芥子碱 小鼠肝癌H22 抗肿瘤 细胞凋亡 BAX/BCL-2 

分 类 号：R285.5[医药卫生—中药学]