脂肪酸受体GPR120与葡萄糖转运蛋白4的关系  

Correlation between fatty acid receptor GPR120 and glucose transporter 4

作　　者：刘丹丹[1] 俞秋霞[1] 张慧娟[1] 姜晓艳[1] 

机构地区：[1]哈尔滨医科大学附属第一医院内分泌科,哈尔滨150001

出　　处：《中国免疫学杂志》2014年第11期1477-1479,1484,共4页Chinese Journal of Immunology

基　　金：黑龙江省卫生厅科研课题(2012-591);哈尔滨医科大学附属第一医院科研基金(2012YB008)资助

摘　　要：目的:研究在3T3-L1细胞中G蛋白偶联受体120(GPR120)与葡萄糖转运蛋白4(GLUT4)的关系。方法:诱导3T3-L1细胞分化,RT-PCR检测GRP120 mRNA表达,油红O染色检测细胞内脂肪;采用siRNA技术下调3T3-L1细胞中GPR120的表达,软脂酸孵育3T3-L1细胞24 h后,用real-time PCR和Western blot方法检测3T3-L1细胞中GLUT4的表达水平的变化。结果:诱导3T3-L1细胞分化过程中GPR120 mRNA表达升高(P<0.05),干扰GPR120表达导致3T3-L1细胞诱导产生的脂滴体积和数量明显减小。另外,干扰GPR120表达导致GLUT4 mRNA和蛋白表达水平下降(P<0.05)。结论:GPR120影响了胰岛素信号通路中GLUT4表达水平,推测其参与了胰岛素抵抗的发生。Objective： To investigate the correlation between G protein-coupled receptor 120（ GPR120） and glucose transporter 4（ GLUT4） in 3T3-L1 cells. Methods： 3T3-l1 cells were induced for differentiation,GRP120 mRNA was detected by RT-PCR and Oil red O was used to determine fat expression. GPR120 expression was knocked down by a specific siRNA in 3T3-L1 cells. 3T3-L1 cell that were tranfected with GPR120 siRNA were incubated with palmitic acid for 24 hours. Then,real-time PCR and Western blot were used to detect the expression of GLUT4 mRNA and protein. Results： Induced differentiation led to GPR120 overexpression in 3T3-L1cells（ P〈0. 05）. GPR120 knockdown resulted in reduction of both lipid volume and number in 3T3-L1 cells. Furthermore,GPR120 knockdown decreased GLUT4 mRNA and protein levels in 3T3-L1 cells（ P〈0. 05,respectively）. Conclusion： GPR120 affects the expression level of GLUT4 in insulin signaling pathway,suggesting its involvement in the initiation of insulin resistance.

关 键 词：游离脂肪酸 葡萄糖转运蛋白4 G蛋白偶联受体120 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]