Ⅰ型IFN体外增强人γδT细胞对骨肉瘤的杀伤作用  

TypeⅠ IFN-mediated enhancement of anti-osteosarcoma cytotoxicity of human gammadelta T cells

作　　者：李朝旭[1] 王锐英[1] 唐际存[1] 

机构地区：[1]桂林医学院附属医院骨二科,桂林541004

出　　处：《中国免疫学杂志》2014年第11期1533-1535,1542,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(81360400)

摘　　要：目的:探讨Ⅰ型干扰素(IFN)对人来源γδT细胞杀伤骨肉瘤作用的影响。方法:取健康人和骨肉瘤患者外周血来源的单个核细胞,分别使用唑来膦酸(Zol)、Zol和Ⅰ型IFN体外刺激扩增,流式细胞技术检测γδT细胞的纯度,计算扩增倍数。以Zol刺激体外扩增的γδT细胞为对照组,Zol联合Ⅰ型IFN刺激体外扩增的γδT细胞为实验组,LDH法检测不同方法扩增的γδT细胞的细胞毒活性,ELISA法检测γδT细胞分泌的IFN-γ。结果:Zol、Zol联合Ⅰ型IFN均可高度选择性扩增健康人和骨肉瘤患者外周血单核细胞中的γδT细胞,扩增后γδT细胞具有杀伤骨肉瘤细胞的能力。Ⅰ型IFN预处理γδT细胞后可显著增强γδT细胞的杀伤活性。结论:Ⅰ型IFN能够促进人γδT细胞的抗骨肉瘤作用。Objective：To investigate the effect of type I IFN on the cytotoxic activity of γδT cells from peripheral blood mono- nuclear cells （PBMCs） of healthy donors and osteosareoma patients stimulated by zoledronate （Zol） and IL-2 against OS. Methods： PBMCs from healthy donors and osteosarcoma patients were stimulated with Zol＋IL-2 or Zol＋IL-2＋ type ] IFN,respectively. After 14 days of culture ,ex vivo expanded γδT cells were assessed by flow cytometry. γδT cell eytotoxicity against target cells was analyzed using a standard lactate dehydrogenase release assay. IFN-γ secreted from γδT cells was determined by ELISA kits. Results： γδT cells from PBMCs of healthy donors and patients with OS were selectively expanded by Zol＋IL-2 or Zol＋IL-2＋ type I IFN in vitro,respectively, and showed cytotoxicity against OS. In addition, γδT cells pre-treated by Type I IFN showed significantly higher cytotoxicity against OS cells. Conclusion： Type I IFN can enhance human γδT cells＇ cytotoxic activity against OS.

关 键 词：骨肉瘤 ΓΔT细胞 干扰素 细胞毒活性 免疫治疗 

分 类 号：R392.12[医药卫生—免疫学]