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作 者:张吉鹍[1,2] 张震宇[3] 吴文旋[4] 李龙瑞 邹庆华[1,2]
机构地区:[1]江西省农业科学院畜牧兽医研究所,江西南昌330200 [2]江西新天地药业有限公司兽药研究院,江西峡江331400 [3]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018 [4]贵州大学动物科学学院,贵州贵阳550025
出 处:《江西农业大学学报》2014年第5期1097-1102,共6页Acta Agriculturae Universitatis Jiangxiensis
基 金:国家自然科学基金(31060313;31160468);贵州省农业科技攻关项目(黔科合NY字(2009)3085)
摘 要:将9只体况良好,体质量相近(41.3±1.2)kg,安装有永久性瘤胃瘘管的成都麻羊半同胞羯羊,随机分成3组,饲以稻草基础日粮,分别补饲25%(MSL25)、50%(MSL50)与75%(MSL75)的苜蓿(MSL),在进行增重性能组合效应研究的同时,采集各组瘤胃液以获取瘤胃原虫和细菌,用4,6-二脒基-2-苯基吲哚(DAPI)标记细菌的荧光强度,进行瘤胃原虫对细菌吞噬作用的组合效应研究。结果表明:1、瘤胃原虫对细菌的吞噬作用。瘤胃原虫的细菌吞噬量与吞噬速率,均随苜蓿补饲水平的增加而减少,当补饲水平达到50%时,趋于平稳。各补饲水平组的吞噬量在60 min达到饱和,此时吞噬量自高到低的排序为MSL25(381.91)>MSL50(377.02)>MSL50(376.47),括号中的数字为吞噬量,单位为cells/cell。2、增重性能。1MSL25、MSL50与MSL75的日增重分别为19.14 g/d、40.40 g/d与49.26 g/d(P<0.05);2MSL25、MSL50与MSL75单位增重所需的饲料分别为44.14、23.69与21.26,其中MSL50与MSL75的组间差异不显著(P>0.05);3MSL25、MSL50与MSL75每公斤增重饲料成本分别为58.49元/kg、48.56元/kg与59.01元/kg,其中MSL25与MSL75的组间差异不显著(P>0.05)。本研究结论,山羊稻草基础日粮补饲50%的苜蓿可提高瘤胃内蛋白微循环效率,改善增重性能,降低饲料成本。Nine healthy wether Chengdu Ma Goats with the same father,weighing (41.3±1.2) kg,were divided into three groups randomly and fed with rice straw ( RS) supplemented with 25%( MSL25) , 50%( MSL50) and 75%( MSL75) alfalfa hay ( Medicago sativa Linn,MSL) respectively to obtain rumen protozoa and bacteria for determination of the phagocytosis of rumen protozoa on bacteria during the study of associative effects on weight gain.The fluorescence intensity of bacteria labeled by DAPA was tested.The results showed that:A.for the phagocytosis of rumen protozoa on bacteria,the grazing quantity and grazing rate decreased with the increasing level of MSL,and then levelled off as the supplemented level of MSL reached 50%.The grazing quantity reached saturation at 60 min for 3 mixed forages and ranked from the greatest to the least as follows:MSL25 (381.91)〉MSL50 (377.02)〉MSL50 (376.47),the figures in parentheses were grazing quantity,and the units were cells/cell.B.for associative effects on weight gains:a.the daily gains of MSL25, MSL50 and MSL75 were 19.14 g/d,40.40 g/d and 49.26 g/d respectively ( P〈0.05);b.the Feed /Gain of MSL25, MSL50 and MSL75 were 44.14,23.69 and 21.26 respectively,no significant difference between MSL50 and MSL75 was observed;c.the feed costs /Gain of MSL25,MSL50 and MSL75 were 58.49 yuan/kg,48.56 yuan /kg and 59.01 yuan /kg respectively,no significant difference between MSL25 and MSL75 was observed.It may be concluded that the efficiency of rumen protein microcirculation and the daily gains of goats can be improved when the rice straw basic diet is supplemented with 50%MSL.
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