油酸对鸭原代肝细胞脂类氧化相关基因表达的影响  

Effects of oleic acid on lipid oxidative gene expression in primary duck hepatocytes

作　　者：刘玮孟 张静 卢立志[3] 王军一 周磊 

机构地区：[1]临沂市动物疫病预防控制中心,山东临沂276000 [2]罗庄区畜牧局,山东临沂276022 [3]浙江省农业科学院畜牧兽医研究所,浙江杭州310021 [4]临沂市畜牧站,山东临沂276000

出　　处：《饲料工业》2014年第22期48-51,共4页Feed Industry

摘　　要：研究采用改良的原位二步灌流法成功分离和培养了鸭原代肝细胞,通过光镜观察细胞形态、电镜观察超微结构,证实培养细胞为肝细胞。在鸭肝实质细胞培养液内加入100μM油酸单体,继续培养48 h后,结果发现,油酸改变了PPARα、ACOX、LPL、L-FABP等脂类氧化相关基因的表达量,其中PPARα、ACOX、LPL基因在添加油酸后显著增加(P<0.05),但是L-FABP基因的表达量却未发生显著变化。PPARα等脂类氧化相关基因表达量的增加,有利于减少脂质沉积,维护脂类代谢的平衡,保持肝细胞的正常生理功能。Hepatocytes were isolated by modifying the current collagenase perfusion method. The cul-tured cells were proved to be hepatocytes by observing morphology and structure under the light mi-croscopy and the electron microscopy. The expression of PPARα and ACOX, LPL, L- FABP werechanged after oleic acid（100 μM） exposure. The expression of the PPARα, ACOX, and LPL genes in-creased significantly following oleic acid exposure（P0.05）, but the expression of L-FABP showed nosignificant change at the end of the experiment. The increasing of lipid oxidative related gene expres-sion, was helpful to reduce lipid deposition, to maintain lipid metabolism balance and maintain the nor-mal physiological function of hepatocytes.

关 键 词：油酸 肝实质细胞 基因表达 鸭 

分 类 号：S816.7[农业科学—饲料科学]