PGC-1α对C2C12成肌细胞增殖的影响  

Influence of PGC-1α on C2C12 myoblast proliferation

作　　者：林亚秋[1] 谈永萍[1] 赵燕英[1] 贺庆华[1] 罗敏[2] 邱翔[1] 钟红梅[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]西南民族大学学报编辑部,四川成都610041

出　　处：《西南民族大学学报（自然科学版）》2014年第6期814-817,共4页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：国家自然科学基金(No.31201990);四川省应用基础项目(2014JY0088)

摘　　要：PGC-1α(Peroxisome Proliferators Activated Receptor gamma coactivator 1 alpha)是一种核受体转录辅助激活因子,广泛参与机体的生物氧化和能量代谢活动.本实验以小鼠C2C12成肌细胞为材料,通过转染pc DNA3.1-flag-PGC-1α真核表达载体,并且与转染空载体的实验对照组比较,利用荧光定量PCR检测转染效果,利用MTT比色法检测PGC-1α对成肌细胞增殖的影响;结果显示,PGC-1α在C2C12成肌细胞中被成功超表达,在转染后的2-5 d,转染真核表达载体组与转染空载体组相比,显著抑制细胞增殖(P<0.05),但在第6 d差异不显著.Peroxisome proliferator-activated receptor co-activator 1α（PGC-1α） is a functional activator of peroxisome proliferator-activated receptor, involved in biological oxidation and energy homeostasis. In the present study, a plasmid pc DNA3.1-flag-PGC-1α was constructed and transfected into C2C12 cells. PGC-1α over-expressions were detected using real-time quantitative PCR. Furthermore, the influence of PGC-1α on C2C12 cell viability was examined by MTT. The results showed that PGC-1α was over-expressed in C2C12 cells, the cell proliferation was significantly inhibited in PGC-1α transfection groups versus control groups（empty vector transfection） in the 2nd to 5th days after transfection（P〈0.05）. However, the inhibition effect was not significant on the 6th day.

关 键 词：PGC-1Α C2C12成肌细胞 增殖 

分 类 号：Q591[生物学—生物化学]