低氧预适应对大鼠肝切除后残肝组织凋亡相关蛋白表达的影响及意义  

Impact and significance of hypoxic preconditioning in the expression of apoptosis-related protein in remaining liver tissue of rats after he- patic resection

作　　者：朱世春[1] 刘歆农[1] 张培建[1] 王健[1] 李鹏飞[1] 韩效帆[1] 

机构地区：[1]扬州大学第二临床医学院普通外科研究室,扬州225002

出　　处：《国际外科学杂志》2014年第11期754-758,F0004,共6页International Journal of Surgery

摘　　要：目的观察低氧预适应对大鼠肝切除术后残肝组织凋亡相关蛋白细胞色素c和caspase3表达的影响及其意义。方法采用SD大鼠肝切除模型研究低氧预适应对残肝组织凋亡相关蛋白表达的影响及意义。将SD大鼠随机分为对照组（NC组）、单纯肝切除组（HR组）和低氧预适应组＋肝切除组（HP组），每组24只。HP组术前用10％氮氧混合气体处理90min，分别于术后1h、6h、12h、24h处死大鼠取肝脏标本，取门静脉血检测肝功能，Western—Blot法测定肝切除大鼠残肝组织细胞色素C和caspase3蛋白的表达，并在电镜下观察各组肝细胞形态学改变、线粒体损伤程度等。结果HP组血清ALT和AST水平显著低于HR组，差异有统计学意义（P〈0．05）；HP组各时段的肝功能明显优于HR组；HP组各时段细胞色素c和caspase3蛋白表达明显低于HR组，透射电镜下HR组肝细胞出现典型的凋亡征象，而HP组肝细胞无明显凋亡形态。结论HP对肝切除后残肝组织的肝细胞凋亡有明显的抑制作用；可能是通过下调细胞色素c和caspase3蛋白的表达、减轻线粒体损伤途径而发挥其保护作用。Objective To explore the impact and significance of hypoxia preconditioning on the expression of cytochrome C and caspase 3 protein in rats after hepatic resection. Methods A hepatectomy model was used to study the ischemia reperfusion injury in hepatic resection. Sprague-Dawley rats were randomly divided into the fol- lowing three groups： normal control （NC） group,hepatic resection（HR） group, and hypoxia preconditioning （HP） group, there were twenty four rats in each group. HP Group was given an 10% oxygen -mixed gas for 90 minutes before the operation. At 1,6, 12 and 24 hours after the operation, the rats were killed and the following tests were conducted ： （ 1 ） Liver tissue was sampled to observe the expression of eytochrome C and caspase 3 protein ; （2） blood was drawn to conduct a chemical examination ; （3） Liver tissue and morphology was observed by transmission electron microscopy. Results The serum levels of ALT and AST in HP group were significantly lower than that of HR group （P 〈0.05） at 1, 6, 12 and 24 hours after the hepatic resection. In each time, liver function of the HP group was significantly better than the HR group; The expression of cytochrome C and caspase 3 protein was de- creased significantly in HP group at each measurement point. Hepatic cells in HR group showed typical apoptosis signs under transmission electron microscopy （TEM） , but no apoptosis was found in HP group. Conclusion HP has marked inhibition to apoptosis by down-regulating the expression of Cyt C and Caspase-3protein and protection to chondrosomes after a hepatic resection.

关 键 词：低氧预适应 肝切除术 细胞色素C类 Caspase 3 细胞保护 

分 类 号：R657.3[医药卫生—外科学]