12份樱桃品种遗传多样性的ISSR分析  

Genetic Diversity Analysis of 12 Cherry Varieties Based on ISSR Marker

作　　者：陈新[1] 张庆霞[1] 徐丽[1] 贾建云[1] 王甲威[1] 刘庆忠[1] 

机构地区：[1]山东省果树研究所/山东省果树生物技术育种重点实验室,山东泰安271000

出　　处：《山东农业科学》2014年第11期12-14,共3页Shandong Agricultural Sciences

基　　金：中俄国际科技合作专项(2012DFR30700);农业部公益性行业(农业)科研专项(No.201203075);"十二五"国家科技支撑计划项目(2013BAD02B03-3-2);山东省农业良种工程项目[鲁科农字(2011)086]

摘　　要：本试验采用ISSR技术对12份樱桃种质资源的遗传多样性进行了研究。筛选出6条ISSR引物对供试材料进行扩增,共检测到48个遗传位点,包含44个多态性位点,多态性位点百分率为91.67%。材料间遗传相似系数为0.29-0.98,平均为0.65。当阈值为0.58时,供试样品可分为3个组,即组Ⅰ、组Ⅱ和组Ⅲ。遗传相似性分析结果显示材料间具有丰富的遗传多样性。The genetic diversity of 12 cherry germplasm resources was analyzed by inter- simple sequence repeat marker in this study.Six primers were selected for the amplification of genome.Forty- eight genetic loci including 44 polymorphic loci were detected with the polymorphic loci percentage as 91.67%.The genetic similarity coefficient ranged from 0.29 to 0.98 with the mean of 0.65.When the genetic similarity coefficient was 0.58,the samples could be divided into three groups,groupⅠ,group Ⅱand groupⅢ.Genetic similarity analysis showed that there were abundant genetic diversities between samples.

关 键 词：樱桃 遗传多样性 ISSR 

分 类 号：S662.501[农业科学—果树学]