不同培养基类型对薄荷组织培养的影响  

Effects of Different Media on Peppermint Tissue Culture

作　　者：张倩[1] 管远清 李青[1] 

机构地区：[1]中国农业大学烟台研究院,山东烟台264670

出　　处：《山东农业科学》2014年第11期30-31,38,共3页Shandong Agricultural Sciences

基　　金：中国农业大学(烟台)科研基金项目(YT201207)

摘　　要：以薄荷茎段为外植体,研究了4种不同类型培养基(MS、1/2MS、B5、N6)对愈伤组织诱导、芽分化以及根诱导的影响。结果表明:1/2MS培养基愈伤组织诱导率最高,为100%,N6培养基愈伤组织的生长量最高;B5培养基芽的诱导率最高,为40%,但1/2MS培养基芽的长势较好;1/2MS培养基最适合根的诱导,诱导率为82.5%。综合分析确定薄荷愈伤组织和根系诱导的最适宜培养基为1/2MS培养基,芽分化的最适宜培养基为B5培养基。Using the stems of peppermint as explants,the effects of 4 different culture media（ MS,1 /2MS,B5 and N6） on callus induction,bud differentiation and root induction were studied in this paper. The results showed that the callus induction rate was the highest in 1 /2MS up to 100%; N6 was the best medium for callus growth; the bud induction rate was the highest in B5 medium as 40%,but the bud grew better in 1 /2MS; 1 /2MS was the most suitable for root induction with the induction rate as 82. 5%. Comprehensive analysis showed that the best medium for peppermint callus and root induction was 1 /2MS,while the best medium for bud induction was B5.

关 键 词：薄荷 组织培养 培养基 诱导率 

分 类 号：S567.235.035.3[农业科学—中草药栽培]