新疆紫草PAL,HMGR,PGT基因的过表达和RNA干扰载体的构建  

Overexpression and RNAi vectors built for key secondary metabolic pathway genes PAL,HMGR,PGT of Arnebia euchroma

作　　者：谢腾[1,2] 刘玉忠[2] 王升[2] 刘谈[2] 康利平[2] 郭兰萍[2] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031 [2]中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京100700

出　　处：《中国中药杂志》2014年第23期4538-4543,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81130070;81072989);国家科技支撑计划项目(2012BAI29B02;2012BAI28B002)

摘　　要：新疆紫草Arnebia euchroma是药用紫草的主要来源,其最重要成分紫草素类化合物具有很高的药用和工业价值。研究采用GATEWAY技术,构建新疆紫草次生代谢关键酶基因PAL,HMGR,PGT的过表达载体和RNAi载体,为相关转基因株系的构建搭建好平台。新疆紫草次生代谢关键酶基因过表达和RNA干扰载体的构建,是基于遗传物质的遗传研究平台的搭建,对于次生代谢途径上基因功能验证和功能基因的挖掘提供了极大的便利,同时也填补了新疆紫草在转基因领域研究的空白,对于培育紫草素高产株系具有重要意义。Arnebia euchroma is the main source for medicinal herb Zicao. and its most important component shikonin compounds have high medicinal and industrial value. This research is aimed to build overexpression vectors and RNAi vectors for key sec- ondary metabolism genes of A. euchroma, and bulid platform for constructions of related transgenic lines using GATEWAY technology. To build genetic material based genetic research platform is to provide a great convenience for digging and functional verification of the genes on secondary＂ metabolic pathway, and also to fill the gaps in transgenic research of A. euchroma. This study is also important for the cultivation of shikonin high-yielding strains of A. euchroma.

关 键 词：新疆紫草 过表达载体 RNAI载体 GATEWAY技术 

分 类 号：R282.71[医药卫生—中药学]