基于SRAP和SNP分子标记的国内穿心莲遗传多样性分析  

Genetic diversity analysis of Andrographis paniculata in China based on SRAP and SNP

作　　者：陈蓉[1,2] 王晓云[3,4,5] 宋毓宁 朱云峰[3,4,5] 王鹏良 李敏[3,4,5] 钟国跃[2,3,4,5] 

机构地区：[1]北京中医药大学,北京100102 [2]中国中医科学院道地药材国家重点实验室培育基地,北京100700 [3]江西中医药大学,江西南昌330004 [4]江西民族传统药现代科技与产业发展协同创新中心,江西南昌330004 [5]江西省中药种质资源工程技术研究中心,江西南昌330004 [6]广西省林业科学院,广西南宁530022

出　　处：《中国中药杂志》2014年第23期4559-4565,共7页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2012BAI29B02)

摘　　要：为了阐明国产穿心莲的遗传多样性以及探讨遗传背景对穿心莲药材质量的影响,利用110对SRAP引物和1对SNP特异性引物,对我国7个省份13个样地的103份穿心莲样品进行遗传多样性分析。采用Powermarker V3.25和Mega6.0软件分析群体的遗传结构,Codon Code Aligner软件分析功能基因单核苷酸多态性变异。聚类分析结果表明各穿心莲样品的遗传距离范围为0.01~0.09,CPS基因片段中未检测到SNP位点,国产各地穿心莲种质间遗传多样性较贫乏,这与穿心莲严格的自花授粉繁殖特性、我国各地引种穿心莲种源来源单一密切相关。遗传背景并非影响各地穿心莲质量差异的主要原因,穿心莲的良种选育以采用突变育种、多倍体育种和分子育种等方法为宜。In order to reveal genetic diversity of domestic Andrographis paniculata and its impact on quality, genetic back- grounds of 103 samples from 7 provinces in China were analyzed using SRAP marker and SNP marker. Genetic structures of the A. paniculata populations were estimated with Powermarker V 3.25 and Mega 6.0 software, and polymorphic SNPs were identified with CodonCode Aligner software. The results showed that the genetic distances of domestic A. paniculata germplasm ranged from 0.01 to 0.09, and no polymorphic SNPs were discovered in coding sequence fragments of ent-copalyl diphosphate synthase. A. paniculata germplasm from various regions in China had poor genetic diversity. This phenomenon was closely related to strict self-fertilization and earlier introduction from the same origin. Therefore, genetic background had little impact on variable qualities of A. paniculata in do- mestic market. Mutation breeding, polyploid breeding and molecular breeding were proposed as promising strategies in germplasm innovation.

关 键 词：穿心莲 SRAP分子标记 SNP分子标记 遗传多样性 

分 类 号：R282.71[医药卫生—中药学]