《中国药典》二氧化硫测定法应用于白芍药材检测的探讨  

Reliability of sulfur dioxide determination method documented in Chinese Pharmacopoeia for evaluating Paeoniae Radix Alba

作　　者：刘欢欢[1,2,3] 孔铭[2,3] 李秀杨 李松林[2,3] 

机构地区：[1]南京中医药大学,江苏南京210046 [2]江苏省中医药研究院 [3]中国中医科学院江苏分院中药分析和代谢组研究室,江苏南京210028

出　　处：《中国中药杂志》2014年第23期4615-4619,共5页China Journal of Chinese Materia Medica

基　　金：国家中医药管理局行业专项(201307008-2);国家高技术研究发展计划(863)项目(2014AA022204);中国中医科学院江苏分院课题(JSBN1301)

摘　　要：为了探讨2010年版《中国药典》第一增补本附录二氧化硫(SO2)残留量测定法应用于白芍药材检测的可靠性,采用药典方法测定白芍药材中SO2残留量,考察方法的重复性;采用HPLC测定白芍残渣和蒸馏液中芍药苷亚硫酸酯的含量,考察芍药苷亚硫酸酯被盐酸置换转化释放SO2的情况。研究发现药典方法检测白芍药材SO2残留量时,结果重复性差;SO2残留检测达"终点"时,药材残渣和蒸馏液中均检测到芍药苷亚硫酸酯,说明芍药苷亚硫酸酯没有完全被盐酸置换成亚硫酸释放SO2。药典方法不能准确检测白芍药材SO2残留量,有必要进行修订或建立新的高选择性检测方法。The content of SO2 in Paeoniae Radix Alba （RPA） was determined by the method documented in Chinese Pharmacopoeia （CP） 2010 edition to validate the repeatability of the method for evaluating RPA, and the contents of paeoniflorin sulfonate in both the residual material and distilled solution of RPA were determined by HPLC to study the transformation of paeoniflorin sulfonate to SO2 by HCl. It was found that the repeatability of the method in CP for evaluating RPA is unacceptable, and paeoniflorin sulfonate was detectable in both the residual material and distilled solution of RPA even at ＂the end point＂ of SO2determination, merely about 50% of paeoniflorin sulfonate was transformed to SO2 by HC1, indicating that the current SO2 determination method in CP is not able to accurately quantify SO2 in RPA. It is recommended that more special method for determining SO2 content in RPA should be developed re- garding the chemical characteristics of sulfur-fumigated RPA.

关 键 词：二氧化硫残留 白芍 HPLC 芍药苷亚硫酸酯 

分 类 号：R284[医药卫生—中药学]