穿心莲二萜内酯有效部位化学成分的液质联用法鉴定及其初步药效学研究  

Identification of main chemical constituents of diterpene lactone effective fraction of Andrographis panniculataby HPLC-DAD /ESI-MS and their preliminary pharmacodynamics research

作　　者：李景华[1,2] 许笑笑 赵炎葱[4] 韩光[1] 

机构地区：[1]河南大学药物研究所,河南开封475004 [2]河南大学天然药物与免疫工程重点实验室,河南开封475004 [3]邓州市中心医院,河南南阳474150 [4]河南大学第一附属医院,河南开封475001

出　　处：《中国中药杂志》2014年第23期4642-4646,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81373974);河南省自然科学基金项目(122102310029);开封市科技关计划项目(1403132)

摘　　要：目的：建立HPLC-DAD-ESI-MS分析鉴定穿心莲二萜内酯有效部位的主要化学成分的方法,并对其药效学进行研究。方法：Agilent SB-C18色谱柱（2.1 mm×150 mm,5μm）;流动相A为乙腈,流动相B为水,60 min梯度洗脱;流速0.4 m L·min-1;进样5μL;检测波长205 nm。柱温为25℃;采用正离子模式,m/z扫描范围50~1 000;利用2种肿瘤细胞株造活体动物模型,对有效部位进行抗肿瘤方面的初步药效学研究。结果：穿心莲二萜内酯有效部位中有5种二萜内酯类化学成分获得了良好的分离与鉴定。药效学实验表明,穿心莲二萜内酯有效部位对肿瘤有一定的抑制作用。结论：建立了一种基于HPLC-DAD-ESI-MS技术对穿心莲二萜内酯有效部位的化学成分进行快速鉴别的方法,并进行了初步药效学研究,为穿心莲二萜内酯有效部位的质量控制及体内的深入研究奠定了基础。Objective： To establish an HPLC-DAD/ESI-MS method for quickly identifying chemical constituents in diterpene lactone effective fraction of Andrographis panniculata and to study its pharmacodynamics. Method ： The separation was performed on an Agilent SB-C18 column （2. 1 mm×150 mm, 5 μm） with a mobile phase of acetonitrile （A） and water（B）. The flow rate was maintained at 0. 4 mL · min- i and detection wavelength was set at 205 nm. The samples were analyzed in positive ion mode, and mass scan range was m/z 50-1 000. Using two kinds of tumor cell lines made living animal models, and studied preliminary pharmacodynamics on anti-tumor aspect. Result： Five diterpene lactones in the diterpene lactone effective fraction of A. panniculata could be separated in one run. Pharmacodynamic experiments showed that the effectve fraction had an inhibitory effect on the growth of tumor. Conclusion ： A rapid and efficient HPLC-ESI-MS method to determine the chemical constituents in diterpene lactone effective fraction of A. panniculata has been established, and the preliminary pharmacodynamics research has been done, which could be used for the quality control and further studies of diterpene lactone effective fraction of A. panniculata in vivo.

关 键 词：液质联用技术 穿心莲二萜内酯有效部位 化学成分 药效学研究 

分 类 号：R284[医药卫生—中药学]