白头翁皂苷影响RA模型大鼠FLS Me CP2表达  

Pulchinenoside control Me CP2 expression in FLS from RA model rats

作　　者：缪成贵[1,2,3] 周国梁[1] 秦梅颂[1] 陈建中[1] 李成凤[1] 何华奇[4] 

机构地区：[1]安徽科技学院食品药品学院,安徽蚌埠233100 [2]安徽医科大学药学院,安徽合肥230032 [3]安徽科技学院家禽疫病监测安徽省重点实验室,安徽蚌埠233100 [4]安徽科技学院生命科学学院,安徽蚌埠233100

出　　处：《中国中药杂志》2014年第23期4664-4668,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81302783);安徽省高校振兴计划人才项目(098);安徽省质量工程项目(2013zy057);滁州市科技局科研项目(201306);安徽省教育厅科研项目(KJ2012Z069);安徽科技学院第十一批校大学生创新课题(14XSF162;14XSZ164)

摘　　要：研究中药白头翁主要活性成分白头翁皂苷(PULC)对类风湿性关节炎(rheumatoid arthritis,RA)模型大鼠Me CP2表达的调控作用。采用佐剂性关节炎大鼠为RA模型,原代培养RA模型大鼠滑膜成纤维细胞样滑膜细胞(FLS),Real time q PCR,Western blotting检测100 mg·kg-1PULC灌胃治疗对RA模型大鼠FLS Me CP2表达的调控作用,MTT检测PULC治疗对FLS增殖的影响,Me CP2 siRNA干扰RA模型大鼠FLS Me CP2表达后SFRP2,β-catenin表达的变化。检测发现经100mg·kg-1PULC灌胃治疗后,RA模型大鼠FLS中Me CP2表达明显降低,SFRP2表达升高,RA模型大鼠FLS增殖活力降低,Me CP2 siRNA抑制RA模型大鼠FLS Me CP2表达后,SFRP2表达明显上调,β-catenin表达明显降低。PULC可能通过抑制Me CP2表达上调SFRP2表达,抑制RA模型大鼠滑膜Wnt通路活性,抑制RA模型大鼠滑膜异常增殖。The role of pulchinenoside （PULC） in the regulation of MeCP2 expression was investigated in RA model rats. Ad- juvant arthritis rats were used as RA model rats, and fibroblast-like synoviocytes （FLS） from the RA model rats were cultured. The, effect of 100 mg ·kg^-1 PULC gavage treatment on the MeCP2 expression and the effect of MeCP2 siRNA on the expression of SFRP2 and β-catenin were detected by real time qPCR and Western blotting. The role of PULC in the FLS proliferation was detected by MTT. The results showed that the MeCP2 expression was down-regulated, the SFRP2 expression was up-regulated and the FLS proliferation was inhibited in FLS after therapy. MeCP2 siRNA significantly inhibited the MeCP2 expression, up-regulated the SFRP2 expression and inhibited the β-catenin expression in FLS from RA model rats. PULC may increase the SFRP2 expression, inhibit the Wnt signaling and inhibit the FLS proliferation in FLS from the RA model rats by inhibiting the MeCP2 expression.

关 键 词：白头翁皂苷 类风湿性关节炎 ME CP2 SFRP2 WNT 

分 类 号：R259[医药卫生—中西医结合] R-332[医药卫生—中医内科学]