交泰丸对db/db小鼠的治疗作用及其脂肪组织PGC-1α、GLUT4基因表达的影响  

Therapeutic Effect of Jiaotai Pill on db/db Mice and Influence on PGC-1α and GLUT4 Gene Expressions in Adipose Tissue

作　　者：汤鹏[1] 胡娜[1] 袁琳[1] 李慧娇[1] 钟霄毓[1] 林敏[1] 孙银强[1] 陆雄[1] 

机构地区：[1]上海中医药大学科技实验中心,上海201203

出　　处：《中国中医药科技》2014年第6期630-632,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：上海市卫生局项目No.20114051;上海市教育委员会预算内科研项目No.2010JW23;上海市教育委员会预算内科研项目No.2011JW18

摘　　要：目的:观察交泰丸对db/db小鼠的治疗作用,并通过观察其对脂肪组织PGC-1α和GLUT4基因表达的影响,探讨其部分作用机制。方法:将db/db小鼠分为模型组、交泰丸组和罗格列酮组,db/m小鼠作为正常对照组。药物灌胃治疗4周,正常组和模型组给予生理盐水。观察体重、饮水量、进食量、尿量变化,观察空腹血糖(FBG)、腹腔注射葡萄糖耐量(IPGTT)和脂肪组织PGC-1α(RT-PCR)、GLUT 4(Western blot)表达的差异。结果:与正常组比较,db/db小鼠组均呈现肥胖、多饮、多食、多尿、高血糖症状;与模型组比较,交泰丸组饮水量、摄食量、尿量、FBG、IPGTT均降低(P<0.05);PGC-1α和GLUT 4表达均增多(P<0.05)。结论:交泰丸对db/db小鼠有较好的降糖效果,降糖机制可能与增加脂肪组织PGC-1α和GLUT 4的表达有关。Objective： To study the effects of Jiaotai pill on type 2 diabetes mellitus db / db mice,explore the part mechanism by observing the influence on PGC-1α and GLUT4 gene expressions in adipose tissue. Methods： db / db mice were randomly divided into model group,Jiaotai pill group,rosiglitazone group and db / m mice were regarded as normal group. Mice in different treatment groups were given corresponding therapy for 4 weeks,mice in normal group and model group were received only physiological saline. During this period,body weight,water intake,food-intake,urine volume,fasting blood glucose（ FBG）,intraperitoneal injections of glucose tolerance（ IPGTT）,PGC-1α（ RT-PCR） and GLUT4（ western blot） expressions were determined after 4 weeks.Results： To compare with normal group,model group mice showed obesity,polydipsia,polyphagia,polyuria,hyperglycemia,to compare with model group,water intake,food intake,urine volume,FBG,IPGTT all had a significant decrease（ P〈0. 05） in Jiaotai pill group; PGC-1α and GLUT4 expressions had a significant increase（ P〈0. 05）. Conclusion： Jiaotai pill is effective for the treatment of type 2 diabetes mellitus mice,the possible mechanism is connected with the increasing of PGC-1α and GLUT4 gene expressions in adipose tissue.

关 键 词：糖尿病 非胰岛素依赖型 交泰丸 PGC-1Α GLUT4 基因表达 DB/DB小鼠 

分 类 号：R285.5[医药卫生—中药学]