超高频超声成像评估动脉粥样硬化小鼠升主动脉弹性属性  

Study on aorta elastic properties in experimental mouse model of atherosclerosis by ultra-high frequency ultrasound

作　　者：刘亚[1] 王俊力[1] 张隽[1] 刘娅妮[1] 邓又斌[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院超声影像科,武汉430030

出　　处：《中华超声影像学杂志》2014年第11期990-993,共4页Chinese Journal of Ultrasonography

基　　金：国家自然科学基金资助（81101053,81371581）;中央高校基本科研业务费专项资金资助（2013QN190）

摘　　要：目的采用超高频超声成像评估实验小鼠动脉粥样硬化病变进程中主动脉弹性属性，探讨主动脉弹性与动脉粥样硬化斑块负荷之间的关系。方法50只高脂饮食喂养的雄性载脂蛋白E基因敲除小鼠作为年龄依赖性动脉粥样硬化病变模型，分别于8、16、24与32周龄，采用超高频超声成像结合测微压力计测算升主动脉弹性模量，同时，超高频超声成像结合组织病理学分析，通过测量主动脉弓小弯侧与无名动脉起始处血管壁厚度、计算斑块与血管面积比值评估升主动脉粥样硬化斑块负荷程度。结果动脉粥样硬化小鼠模型中，从8周龄至32周龄，主动脉弓小弯侧及无名动脉起始处血管壁厚度以及斑块与血管面积的比值呈进行性增加；同时，主动脉弹性模量值亦呈年龄依赖性增加；各组间指标差异具有统计学意义。结论随着动脉粥样硬化性病变的进展以及斑块负荷的加重，主动脉弹性属性呈进行性减低；超高频超声成像能够清晰显示小鼠主动脉结构，是一种可靠的评估动脉粥样硬化斑块严重程度以及主动脉弹性属性的检查方法。Objective To assess the aorta elastic properties in the procession of atherosclerosis by ultra-high frequency ultrasound,and to detect the relationship between the aorta elastic properties and the atherosclerotic plaque burden. Methods Mice deficient for the apolipoprotein E （ApoE-/-） with high- cholestrol diet were studied as an age-dependent model of atherosclerosis.At 8,16,24 and 32 weeks of age, the blood pressure in the ascending aorta was measured by catheter, and the aorta mechanical properties were assessed by measuring aortic elastic modulus of the ascending aorta with ultra-high frequency ultrasound.The plaque burden was assessed by high-frequency ultrasound and Masson＇s trichrome stain, separately. Results Vessel thickness at the lesion-prone sites of the lesser curvature of the aorta and the proximal brachiocephalic artery increased with age,consistent with the Massonrs trichrome staining which showed age-dependent worsening of atherosclerosis in the mice model. Elastic modulus of the aorta significantly increased from 8 to 32 weeks of age in E-/- mice.There was a statistically difference between any two groups.Conclusions With the progression of atherosclerosis and the increased plaque burden,aorta mechanical properties deteriorated in Apo E-/-mice.Ultra-high frequency ultrasound was a potential tool for assessment of plaque burden and aorta mechanical properties in mouse model.

关 键 词：超声检查 动脉粥样硬化 主动脉 弹性 

分 类 号：R543.5[医药卫生—心血管疾病]