6-OHDA损伤神经细胞CXCR4/CXCL12表达变化的实验研究  

The change of PC12 cells and the expression of CXCR4/CXCL12 under the 6-OHDA's injury

作　　者：王辉[1] 暴丽华 李涛[3] 李贵宝[1] 吴琦[1] 朱德晓[1] 吴金涛[1] 刘海莉[1] 张静[1] 孙晋浩[1] 

机构地区：[1]山东大学医学院人体解剖学教研室,济南250012 [2]长治市人民医院神经内科,山西长治046000 [3]济南市第四人民医院神经外科,济南250030

出　　处：《中国临床解剖学杂志》2014年第6期680-684,共5页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金(81371471;81071081);教育部博士点基金(20120131120049);山东省自然基金(ZR2012HQ018;ZR2010HM051);山东省科技发展计划(2011GSF11810);山东大学自主创新基金(2012TS113);山东省卫生厅基金(2013WS0008)

摘　　要：目的研究6-OHDA损伤PC12细胞CXCR4/12表达的变化。方法以梯度浓度的6-OHDA处理PC12细胞,光镜观察各组PC12细胞的形态学变化;MTT法检测6-OHDA对PC12细胞的损伤;间接免疫荧光染色法检测6-OHDA不同浓度下PC12细胞CXCR4/CXCL12表达的变化。结果随6-OHDA浓度的增加,PC12细胞的存活率呈梯度降低;光镜下观察发现PC12细胞数减少,轴突变短甚至消失;间接免疫荧光染色则显示CXCL12及其受体CXCR4在神经细胞的表达增加。结论 6-OHDA浓度的增加可导致PC12细胞凋亡,同时伴有CXCL12及其受体CXCR4表达增加。Objective To study the change of PC12 cells and the expression of CXCR4 and CXCL12 under the 6-OHDA＇ s injury. Methods PC12 cells were treated with concentration gradient of 6-OHDA. The the morphological change of PC 12 cells was observed with light microscope, then MTT assay was used to detect the viability of PC12 cells treated with 6-OHDA. And the expression of CXCL12 and CXCR4 was detected in different 6-OHDA concentrations with indirect immunofluorescence technique. Results With the increase of the concentration of 6-OHDA, the survival rate of PCI2 cells decreased gradually. The number of the PC12 cells decreased, with the axon becoming shorter or disappearing. The results of indirect immunofluorescence technique showed that the expression of CXCR4 and CXCL12 increased with the amount of 6-OHDA. Conclusion The increase of the concentration of 6-OHDA leads to the apoptosis of the PC 12 cells and the increase of the expression of CXCR4 and CXCL 12.

关 键 词：6-OHDA CXCR4/CXCL12 PC12细胞 形态学变化 间接免疫荧光染色 

分 类 号：R965[医药卫生—药理学]