枸杞多糖对癫痫大鼠海马神经细胞增殖的影响  

Effect of Lycium barbarum polysaccharide influence nerve cell proliferation in hippocampus of epilepsy rat

作　　者：张义伟[1] 马江波[2] 孙金萍[3] 刘印明[2] 周惠惠[2] 张潇剑[2] 刘娟[1] 

机构地区：[1]宁夏医科大学颅脑疾病重点实验室,银川750004 [2]宁夏医科大学基础学院人体解剖与组织胚胎学系,银川750004 [3]宁夏医科大学总医院病理科,银川750004

出　　处：《神经解剖学杂志》2014年第6期693-696,共4页Chinese Journal of Neuroanatomy

基　　金：宁夏医科大学博士点建设开放课题;宁夏自然科学基金(NZ13272)

摘　　要：目的:探讨枸杞多糖(Lycium barbarum polysaccharide,LBP)对大鼠癫痫发作后海马齿状回颗粒细胞层神经细胞的影响。方法:选择健康5周龄雄性SD大鼠,随机分为三组,即对照组、癫痫组和LBP干预组。癫痫组腹腔注射氯化锂-匹罗卡品,LBP干预组在注射氯化锂-匹罗卡品前分别灌服25 mg/kg、50 mg/kg、100 mg/kg的LBP 2周,对照组给予等剂量生理盐水。溴脱氧尿嘧啶核苷(Brd U)标记后免疫荧光染色,观察大鼠海马齿状回颗粒细胞层Brd U阳性细胞的增殖情况。结果:与对照组相比较,癫痫组的Brd U阳性细胞明显增多(P<0.01);与癫痫组相比较,不同剂量LBP干预组的Brd U阳性细胞均明显减少(P<0.01),但各剂量LBP干预组间无显著性差异(P>0.05)。结论:LBP可干预大鼠癫痫发作后海马齿状回颗粒层Brd U阳性细胞数的异常增生,具有明显的神经保护作用。Objective：To investigate the influence of Lycium barbarum polysaccharide（ LBP） on the nerve cells of hippocampal dentate gyrus granular cell layer in epilepsy rat.Methods：Male Sprague-Dawley rats（ 5 weeks） were randomly assigned into three groups：control group,epilepsy group,and intervention group.Lithium-pilocarpine was injected into the abdominal cavity to create epilepsy model in rats.Intervention group rats were administered with LBP（ 25 mg / kg,50 mg / kg,100 mg / kg） via oral gavage once a day for two weeks,and then injected Lithium-pilocarpine,while the control group received only same volume of saline.After the treatment,the number of 5-bromodeoxyuridine（ Brd U） labeling of positive cells was observed by immunofluorescence staining.Results：The levels of Brd U positive cells are remarkably enhanced in epilepsy group compared with control group（ P〈0.01）.The number of Brd U positive cells in animals treated orally with LBP significantly decreased compared to epilepsy group（ P〈0.01）.But withdrawal of oral treatment with LBP was associated with non-significant alterations in other indicators（ P〉0.05）.Conclusion：LBP can decrease the abnormal proliferation of Brd U positive cells of hippocampal dentate gyrus granular cell layer in epilepsy rat.The result may support the fact that LBP has obvious nerve protective effect.

关 键 词：癫痫 枸杞多糖 Brd U阳性细胞 海马齿状回 大鼠 

分 类 号：R285.5[医药卫生—中药学]