丹酚酸B对培养的新生大鼠海马神经元的影响  

Effect of salvianolic acid B on cultured neonatal rat hippocampal neurons

作　　者：杨赣军[1] 张小强[1] 孙弋[1] 

机构地区：[1]井冈山大学医学院,吉安343000

出　　处：《神经解剖学杂志》2014年第6期705-708,共4页Chinese Journal of Neuroanatomy

摘　　要：目的:本研究旨在观察丹酚酸B对体外培养新生大鼠海马神经元的影响,为丹酚酸B对中枢神经系统的作用提供实验依据。方法:体外培养新生大鼠海马神经元,神经元微管相关蛋白-2(MAP-2)免疫荧光染色对培养细胞进行鉴定,实验分为对照组和给药组(终浓度分别为5 mg/L和10 mg/L),荧光显微镜下观察细胞形态,MTT比色法检测丹酚酸B对海马神经细胞的活性,细胞核染色(DAPI)观察丹酚酸B对海马神经细胞增殖的影响。结果:免疫荧光染色显示细胞呈MAP-2阳性,丹酚酸B作用7 d后,能明显促进海马神经细胞的存活与增殖,且10 mg/L的丹酚酸B作用更明显。结论:丹酚酸B能明显促进海马神经细胞的存活与增殖,提示其具有改善脑功能的重要作用。Objective：To explore effect of salvianolic acid B on cultured hippocampal neurons in neonatal rat and provide experiment basis for role of salvianolic acid B on central neural system.Methods：The neonatal rat hippocampal neurons were cultured in vitro and identified by the neuronal microtubule associated protein（ MAP-2） immunofluorescence labeling.The experiments were randomly divided into control group and salvianolic acid B group（ 5 mg / L and 10 mg / L,respectively）.The cellular morphology was observed by fluorescence microscope.The neurons viability was determined by MTT assay.DAPI staining was used to detect the proliferation of cultured neurons.Results：Immunofluorescent staining showed that the cells were MAP-2 positive.Compared with the control group,the hippcampal neurons viability and proliferation in the salvianolic acid B were significantly increased on 7 days,and the effect was more obvious with 10 mg / L salvianolic acid B.Conclusion：The salvianolic acid B can obviously promote hippocampal neurons viability and proliferation,indicating the important role of the salvianolic acid B on improving brain function.

关 键 词：丹酚酸B 海马细胞 MTT 大鼠 

分 类 号：R285.5[医药卫生—中药学]