Menin对MyD88表达调控的初步研究  

Role of Menin in the Regulation of MyD88 Gene Expression

作　　者：张翠平[1] 喻明[1] 张宏利[2] 王晓[2] 李文毅[2] 李果[2] 

机构地区：[1]上海中医药大学附属普陀医院内分泌科,上海市200062 [2]上海市内分泌代谢病研究所,上海交通大学医学院瑞金医院内分泌代谢科,上海市内分泌代谢病临床医学中心,上海市200025

出　　处：《医学分子生物学杂志》2014年第6期331-334,共4页Journal of Medical Molecular Biology

基　　金：国家自然科学基金（No.30771021,30800537）

摘　　要：目的探讨menin蛋白对髓样分化因子88（myeloid differentiation factor 88，MyD88）基因表达的调控作用。方法在Menl基因敲除的小鼠mef（Menl^-/- mef）细胞中，转染可以过表达menin蛋白的menin-PCI-NEO质粒，运用Western印迹技术检测menin对MyD88蛋白表达的影响。构建包含MyD88启动子的报告基因质粒,在Menl^-/- mef细胞中，将MyD88启动子质粒和menin-PCI-NEO质粒或其阴性对照PCI-NEO共转染，利用荧光报告基因系统分析menin对MyD88基因启动子活性的影响。结果转染menin—PCI—NEO质粒后，menin蛋白表达升高的同时。MyD88蛋白的表达减少。在荧光报告基因系统中，转染menin-PCI-NEO质粒后MyD88基因启动子活性被抑制。结论menin蛋白可以下调MyD88蛋白的表达，这种作用可能通过抑制MyD88基因的转录而实现。Objective To explore the role of menin, the protein product of the Menl gene, in the regulation of myeloid differentiation factor 88 （MyD88） gene expression. Methods The plasmid menin-PCI-NEO that can overexpress menin protein was transfected into Menl^-/- mef cells of mouse. Western blotting was performed to examine the effect of menin on the expression of MyD88. Reporter gene vectors with MyD88 promoter were constructed and co-transfected into Menl^-/- mef cells along with menin-PCI-NEC and negative control PCI-NEO, and the influence of menin on the transcription activity of MyD88 promoter was analyzed by dual-luciferase reporter gene assay system. Results Western blotting showed that menin was increased while MyD88 decreased after transfection of menin-PCI-NEO plasmids. Additionally, dual-luciferase reporter gene assay system revealed that the activity of the MyD88 gene promoter was significantly inhibited after the transfection. Conclusion Menin may inhibit the expression of MyD88 protein by inhibiting the transcription of MyD88 gene.

关 键 词：MENIN 髓样分化因子88(MyD88) 表达调控 

分 类 号：Q78[生物学—分子生物学]