HPLC法测定肠溶交泰胶囊中盐酸小檗碱的含量  

Content determination of berberine hydrochloride in Jiaotai enteric coated capsules by HPLC

作　　者：曹臣[1] 黄开颜[1] 李珊[1] 陈迎春[1] 刘绍贵[1] 

机构地区：[1]湖南中医药大学第一附属医院,410007

出　　处：《中国现代药物应用》2015年第2期221-223,共3页Chinese Journal of Modern Drug Application

基　　金：国家中医药管理局名老中医药专家传承工作室建设项目

摘　　要：目的 建立肠溶交泰胶囊中盐酸小檗碱的含量测定方法。方法 采用高效液相色谱法（HPLC）。色谱柱：SHIMADZU VP-ODS 150 mm×4.6 mm;流动相：以乙腈-0.05 mol/L磷酸二氢钾溶液（25∶75）;检测波长：345 nm;流速：1.0 ml/min;柱温：25℃。结果 盐酸小檗碱在0.03012~0.6024μg范围内与峰面积有良好的线性关系,其回归方程为：Y=167557X＋60945,R=0.9999。平均回收率为98.60%,RSD=1.51%。结论 本方法操作简便,重现性好,精密度好,可以用来控制本制剂的质量。Objective To establish a method for content determination of berberine hydrochloride in Jiaotai enteric coated capsules. Methods High performance liquid chromatography (HPLC) was applied by chromatographic column as SHIMADZU VP-ODS 150 mm×4.6 mm with mobile phase as acetonitrile-0.05 mol/L potassium dihydrogen phosphate (25:75). The detection wavelength was 345nm with 1.0ml/min of flow rate and 25℃of column temperature. Results The berberine hydrochloride showed a good linear correlation within the concentration of 0.03012~0.6024μg, and its regress equation was Y=167557X+60945, R=0.9999. The average recovery rate was 98.60%, RSD=1.51%. Conclusion This method has the advantages of simple operation, good reproducibility and precision. It can be used for quality control of this preparation.

关 键 词：肠溶交泰胶囊 盐酸小檗碱 含量测定 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]