补阳还五汤精简方对局灶性脑缺血大鼠脑谷胱甘肽抗氧化系统的影响  

Effects of Thin Recipe of Buyang Huanwu Decoction on Expressions of GSH,GSH-Px and γ-GCS in the Brain of Focal Cerebral Ischemia Rats

作　　者：佘颜[1] 易健[2] 邵乐[2] 夏相宜 刘芳[1] 谭周进[1] 蔡光先[2] 王宇红[2] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学省部共建中医内科学教育部重点实验室,湖南长沙410007

出　　处：《中国中医药信息杂志》2015年第1期58-61,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(2012CB723503);湖南省科技计划项目(2013SK3107);湖南省中药粉体与创新药物省部共建国家重点实验室培基地开放基金(ZYFT201302)

摘　　要：目的观察补阳还五汤精简方对局灶性脑缺血大鼠脑谷胱甘肽抗氧化系统的影响,探讨其作用机制。方法将120只SD大鼠采用随机数字表法分成假手术组、模型组、补阳还五汤组、补阳还五汤精简方组。除假手术组外,其余各组采用大脑中动脉线栓法建立局灶性脑缺血模型,造模2 h后,各给药组给予相应药物灌胃,假手术组、模型组给予等量生理盐水灌胃。各组分别于脑缺血后1、3、7 d检测脑组织还原型谷胱甘肽(GSH)含量、谷胱甘肽过氧化物酶(GSH-Px)活性。同时,采用实时荧光定量PCR、Western blot检测大鼠脑缺血后γ-谷氨酰半胱氨酸合成酶(γ-GCS)m RNA和蛋白的表达。结果与假手术组比较,模型组脑组织GSH含量及GSH-Px活性明显降低(P<0.05,P<0.01),γ-GCS m RNA及蛋白表达升高,其中第1日最明显(P<0.05);与模型组比较,各给药组多能不同程度升高GSH含量及GSH-Px活性,同时上调γ-GCS m RNA及蛋白表达,其中第3日差异有统计学意义(P<0.05)。结论补阳还五汤精简方通过调控脑缺血后谷胱甘肽抗氧化系统中GSH、GSH-Px及γ-GCS的表达,发挥抗氧化作用。Objective To observe the effects of thin recipe of Buyang Huanwu Decoction on expressions of glutathione （GSH） antioxidant system including GSH, GSH-Px and У-GCS in the brain of focal cerebral ischemia rats; To study its mechanism of antioxidant effect. Methods Totally 120 SD rats were randomly divided into four groups according to random number table method： sham-operation group, model group, Buyang Huanwu Decoction group and thin recipe of Buyang Huanwu Decoction group. Except for the sham-operation group, the rest groups established focal cerebral ischemia rat model by middle cerebral arterial occlusion. The treatment groups were given corresponding medicine by gavage, while the sham-operation group and model group were given the same amount of normai saline 2 h after modeling. Each group was detected after cerebral ischemia for 1 day, 3 days, and 7 days respectively. The content of GSH and the activity of GSH-Px were detected. At the same time, Effects of Thin Recipe of Buyang Huanwu Decoction on Expressions of GSH, GSH-Px and У-GCS in the Brain of Focal Cerebral Ischemia RatsGCS mRNA and protein levels were determined by using real time-PCR and Western blot. Results The content of GSH and the activity of GSH-Px at each time point decreased in the model group （P〈0.05）, compared with the corresponding sham-operationgroup. But the expression of У-GCS mRNA and protein increased, with statistical significance on day 1 （P 〈 0.05）. Compared with model group, content of GSH and GSH-Px activity levels increased differently in each treatment group, while y-GCS mRNA and protein expression raised, with statistical significance on day 3 （P〈0.05）. Conclusion The thin recipe of Buyang Huanwu Decoction plays antioxidant effect by regulating the expression of glutathione antioxidant system GSH, GSH-Px and У-GCS after cerebral ischemia, which may be one of its therapeutic mechanisms for cerebral ischemia.

关 键 词：补阳还五汤精简方 局灶性脑缺血 氧化应激 还原型谷胱甘肽 谷胱甘肽过氧化物酶 γ-谷 氨酰半胱氨酸合成酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]