糖网明目颗粒对糖尿病大鼠视网膜病变防治作用及机制研究  

Preventive and Therapeutic Effects and Mechanism of Tangwang Mingmu Granule on Rats with Diabetic Retinopathy

作　　者：蒿长英[1] 陈明霞 郭平 刘国瑞[1] 刘晔 连增林 

机构地区：[1]汉典集团北京红太阳药业有限公司,北京100103 [2]汉典集团北京汉典制药有限公司,北京100103 [3]汉典集团北京汉典中医研究院,北京100103

出　　处：《中国中医药信息杂志》2015年第1期62-66,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京市科技计划"十病十药"中药专项(Z121102001112007)

摘　　要：目的探讨糖网明目颗粒新工艺提取物防治糖尿病视网膜病变的作用机制。方法改进传统水煎煮中药制备工艺,优选提取制备新工艺,采用高效液相色谱法检测2种工艺糖网明目颗粒提取物有效成分的含量;建立体内外药效学评价模型,评价药物作用效果。体外实验采用MTT法观察2种工艺不同浓度的糖网明目颗粒提取物对人脐静脉内皮细胞EA.hy 926增殖作用的影响。体内实验采用视网膜铺片及ELISA观察2种工艺糖网明目颗粒提取物对STZ造模制备的糖尿病大鼠视网膜微血管病变及其相关因子的影响。结果与传统工艺比较,糖网明目颗粒新工艺提取物所含有效成分明显提高;糖网明目颗粒新工艺提取物能够明显改善糖尿病大鼠视网膜微血管病理学状态,降低血管生成相关因子的蛋白水平。结论新工艺能够最大限度地提取和富集糖网明目颗粒中有效成分;新工艺提取物能够明显改善糖尿病大鼠视网膜微血管病理状态,其作用机制可能与调节血管细胞黏附分子及炎性因子有关。Objective To observe the preventive and therapeutic eltects of the new process extracts of Tangwang Mingmu Granule on rats with diabetic retinopathy. Methods The best preparation process technology of drug extraction was improved and selected based on the traditional water decoction of Chinese medicine. The active ingredient of two processes were detected and compared by HPLC. In vitro and in vivo pharmacodynamic evaluation models were used to evaluate the drug effects and analyze its mechanism. MTT assay was used to analyze the inhibitory effects of extracts in the two processes on EA.hy 926 cell proliferation in vitro. The effects of two extracts on STZ induced rat diabetic microvascular disease and its related factors were observed by retinal slice staining and ELISA. Results Compared with the traditional process, the active ingredients of the extracts were improved by the new technology of Tangwang Mingmu Granule. The preparation process of Tangwang Mingmu Granule could significantly improve the diabetic rat retinal microvascular pathological state, and reduce the protein expression levels of angiogenesis related factors. Conclusion The new preparation process could effectively improve the active ingredients in the herbal extract. The extract of new technology showed a significantly effect on the diabetic rats in retinal microvascular pathology state. The mechanism may be related to the regulation ofvascular cell adhesion molecules and inflammatory factors.

关 键 词：糖网明目颗粒 糖尿病视网膜病变 人脐静脉内皮细胞 黏附分子 炎性因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]