正交试验法优选金枣远志胶囊中贯叶金丝桃等提取工艺  

Optimization of Extraction Technology of Hyperici Perforate Herba,Coptidis Rhizoma,Schisandrae Chinensis Fructus,and Ziziphi Spinosae Semen for Jinzao Yuanzhi Capsule by Orthogonal Test

作　　者：李磊[1] 李宏力[1] 姚宏 

机构地区：[1]陕西能源职业技术学院医学技术系,陕西西安710613 [2]西安万隆制药股份有限公司技术中心,陕西西安710119

出　　处：《中国中医药信息杂志》2015年第1期91-94,共4页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的优选金枣远志胶囊中贯叶金丝桃、黄连、五味子、酸枣仁共同提取的工艺。方法以金丝桃苷提取率为考察指标,采用高效液相色谱法进行测定。采用正交试验与比较试验相结合的方法,对提取工艺中的乙醇浓度、浸泡时间、回流提取时间等因素进行优选。结果乙醇浓度及提取次数对考察指标有显著影响。确定金枣远志胶囊的最佳提取工艺为:加8倍量70%乙醇,浸泡2 h,回流提取1.5 h,过滤,残渣再加6倍量70%乙醇回流提取1.5 h,过滤,合并提取液。结论优选的提取工艺合理可行。Objective To optimize the extraction conditions of Hyperici Perforate Herba, Coptidis Rhizoma, Schisandrae Chinensis Fructus, and Ziziphi Spinosae Semen for Jinzao Yuanzhi Capsule. Methods Hyperin extraction rate was set as index, and HPLC was used for determination. The extraction conditions of ethanol concentration, ethanol quantity, extraction time and other factors were studied with orthogonal test and comparative test. Results Ethanol concentration and extracting times were remarkable factors in the test. The optimum extraction technology for Jinzao Yuanzhi Capsule was to reflux and extract the materials with 8 times of 70% ethanol for 2 times, reflux extraction for 1.5 hours, filtration. Then 6 times of 70% ethanol was added for 1.5 hours＇ reflux, filtering, and merging extracted liquid. Conclusion The optimum extraction technology is reasonable and feasible.

关 键 词：金枣远志胶囊 正交试验 提取工艺 贯叶金丝桃:黄连 五味子 酸枣仁 

分 类 号：R283.5[医药卫生—中药学]