入境外籍人员抗-HCV、HCV-RNA与ALT相关性及监测分析  

Correlation analysis and epidemiological surveillance of anti-HCV,ALT,and HCV-RNA of entry expatriates in China

作　　者：石莹[1] 杨雨[2] 田绿波[2] 陈肖潇[2] 高国龙[2] 樊学军[2] 贺黎铭 栾荣生[1] 

机构地区：[1]四川大学华西公共卫生学院(华西第四医院),四川成都610041 [2]四川出入境检验检疫局国际旅行卫生保健中心,四川成都610041

出　　处：《现代预防医学》2015年第1期168-171,共4页Modern Preventive Medicine

基　　金：国家质检公益性科研项目(201210046);国家质检总局科研项目(2013IK242);四川出入境检验检疫局科研项目(SK201302)

摘　　要：目的掌握四川15岸入境外籍人群HCV感染情况及流行病学特征。探讨广东、深圳、云南、四川、辽宁15I岸2010—2012年入境外籍人员抗-HCV阳性人员的抗-HCV、HCV—RNA载量及ALT三者的相关性，对15岸入境外籍人员更科学合理有效地开展丙肝传染病监测起好指导作用。方法对2010—2012年监测的四川15岸入境外籍人群HCV阳性检出者流行病学资料进行统计学分析。收集五大国境口岸保健中心筛查的235例抗-HCV阳性的入境外籍人员血清样本，用酶联免疫吸附测定（ELISA）法进行抗-HCV复检，用实时荧光定量PCR法检测HCV—RNA，同时收集其丙氨酸氨基转移酶（ALT）检测数据。数据采用SAS9．13处理。结果2010—2012年监测的四川口岸入境外籍人群HCV感染率是2．8％。，51～60岁年龄组，来华留学人员感染率最高。收集的235例抗-HCV阳性入境外籍人员血清标本复检后231例阳性，4例阴性。检出HCV—RNA阳性131例（占56．7％），其中ALT异常89例（占67．9％）。100例HCV—RNA阴性中ALT异常25例（占25．0％）。随着HCV—RNA载量的升高，ALT异常率有增高趋势（X^2=44．75，P〈0．01）。同时，ALT的均值随HCV—RNA载量的增加而升高（r=0．973，P〈0．01）。结论HCV—RNA检出率与方法学敏感性、HCV感染所处的临床阶段、抗病毒药物的应用密切相关。HCV—RNA载量与ALT水平呈明显相关性。入境外籍人群中51．60岁年龄组，来华留学人员阳性检出率最高，提示在今后口岸HCV防控与分子流行病学分析工作中，可考虑将其作为重点监测对象。Objective To monitor the prevalence and epidemic of HCV among the entry expatriates, and also to investigate the relationship between Anti-HCV, HCV-RNA loads and the levels of ALT, detect the HCV infections more effectively. Methods The epidemiologieal surveillance of the HCV infection who were monitored through Sichuan ITHC in entry expatriates were analyzed. From 2010 to 2012, 235 positive Anti-HCV samples were collected from Guangdong, Shenzhen, Yunnan, Sichuan and Liaoning international travel heahhcare center （ITHC）. Anti-HCV were retested by ELISA in Sichuan ITHC, the load of HCV-RNA were detected by FQ-PCR and the datas of ALl＂ value were collected from these ITHC. The data were analyzed by SAS 9.13. Results Anti-HCV positive rate was 2.8%e at Sichuan port in entry expatriates, 51 - 60 age group, overseas students were at the highest risk. 131 samples showed HCV-RNA positive （56.7%） in 231 samples which were anti-HCV positive, and 89 samples showed the level of ALT was abnormal in HCV-RNA positive cases. Both the abnormal rate of ALT and the average of ALT value increased with the HCV-RNA load increased. Conclusion The methodological sensitivity, the period of HCV infection and the application of antiviral drugs affected the positive rate of HCV-RNA significantly. HCV-RNA loads and ALT levels showed significantly correlation. The HCV surveillance should focus on 51 - 60 age group and overseas students.

关 键 词：入境外籍人员 抗-HCV阳性 HCV—RNA含量 谷丙转氨酶(ALT) 监测 

分 类 号：R115[医药卫生—公共卫生与预防医学]