尾悬吊模拟失重大鼠胃黏膜超微结构改变及氧化应激损伤  

Ultrastructural changes and oxidative stress injury in gastric mucosa of tail-suspended rats under simulated weightlessness

作　　者：郭彪[1] 李正鹏[1] 周金莲[2] 杨建武[1] 赵燕[1] 刘子沛[1] 付晓艳[1] 董满库[1] 崔彦[1] 

机构地区：[1]解放军第三0六医院普通外科,北京100101 [2]解放军第三0六医院病理科,北京100101

出　　处：《中华生物医学工程杂志》2014年第3期179-184,共6页Chinese Journal of Biomedical Engineering

基　　金：全军医学科研“十二五”重点课题（BWS11J051）

摘　　要：目的 探讨尾悬吊模拟失重对大鼠胃黏膜组织超微结构和氧化应激的影响.方法 健康雄性Wistar大鼠88只,按随机数字表分为11组（n=8）,采用尾悬吊法建立模拟失重大鼠动物模型,各组大鼠分别尾悬吊0h（正常对照组）、6h、12h、1d、2d、3d、5d、7d、14d、21 d、28 d.实验结束后观察各组大鼠胃黏膜组织病理改变,检测胃黏膜组织超氧化物歧化酶（SOD）、丙二醛（MDA）和一氧化氮（NO）含量,免疫组化法检测胃黏膜组织诱生型一氧化氮合酶（NOS2）和环氧化酶2（COX2）的表达.结果 透射电镜下可见尾悬吊模拟失重大鼠胃黏膜部分腺细胞核皱缩畸形,染色质浓缩边聚,线粒体肿胀,嵴断裂、溶解,空泡变性,粗面内质网扩张.上述改变在尾悬吊14d、21d、28 d比较明显.与正常对照组比较,尾悬吊1d、5d、14 d、21 d、28 d组大鼠胃黏膜组织SOD含量明显升高（均P＜0.05）;尾悬吊12h、14d、21 d、28 d组大鼠胃黏膜组织MDA含量明显升高（均P＜0.05）;尾悬吊12h、2d、3d、5d、14d、28d组大鼠胃黏膜组织NO含量明显升高（均P＜0.05）.免疫组化显示,尾悬吊6h、12h、1d、2d大鼠胃黏膜组织NOS2和COX2表达明显增强,随后表达减弱;尾悬吊14d、21 d、28 d表达再度增强.结论 尾悬吊模拟失重可导致大鼠胃黏膜组织超微结构改变和NOS2、COX2表达变化.Objective To investigate the effect of simulated weightlessness on ultrastructural changes and oxidative stress in gastric mucosa of tail-suspended rats.Methods Eighty-eight healthy male Wistar rats were divided into 11 groups （n=8 each） by random number table,and tail-suspension was used to simulate the weightlessness in animal models.The duration of tail-suspension in each group of rats was 0 h （normal control group）,6 h,12 h,1 d,2 d,3 d,5 d,7 d,14 d,21 d and 28 d respectively.After theexperiment,the pathologic changes of gastric mucosa in rats of each group were studied,and the content ofsuperoxide dismutase （SOD）,malondialdehyde （MDA） and nitric oxide （NO） in the gastric mucosa were detected.Immunohistochemical assay was used to examine the expression of inducible nitric oxide synthase （NOS2） and cyclooxygenase 2 （COX2）.Results Transmission electron microscopy revealed nuclear shrinkage of gland cells,condensation of chromatin around the perinuclear membrane,mitochondrial swelling,rupture and lysis of cristae,vacuolar degeneration and dilatation of the rough endoplasmic reticulum in gastric mucosa of tail-suspended rats under simulated weightlessness.These changes were significant in groups of 14 d,21 d and 28 d suspension.Compared with the normal control group,the content of SOD increased obviously in the gastric mucosa of rats with tail-suspension for 1 d,5 d,14 d,21 d and 28 d （all P＜0.05）,as did the content of MDA in those for 12 h,14 d,21 d,28 d （all P＜0.05）,and the content of NO in those for 12 h,2 d,3 d,5 d,14 d and 28 d,respectively （all P＜0.05）.Immunohistochemistry showed enhanced expression of both NOS2 and COX2 in the gastric mucosa of rats with tail suspension for 6 h,12 h,1 d,and 2 d,which then declined until resurging on 14 d,21 d and 28 d.Conclusion Simulated weightlessness can induce ultrastructural alteration,changes of NOS2 and COX2 expressions in gastric mucosa of tail-suspended rats.

关 键 词：失重模拟 大鼠 胃黏膜 细胞结构 应激 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]