长爪沙鼠不同willis血管类型全基因组文库构建  

Genomic library construction of different willis circle in Meriones unguiculatus

作　　者：张贺[1] 陈振文[2] 王承利[1] 孙倩[1] 陆娜[1] 王洋[1] 

机构地区：[1]沈阳军区总医院实验动物科,沈阳110015 [2]首都医科大学实验动物部,北京100669

出　　处：《中国比较医学杂志》2014年第11期27-32,共6页Chinese Journal of Comparative Medicine

基　　金：国家科技支撑计划(2009BAI83B02-41);辽宁省科技攻关项目(2012408002)

摘　　要：目的为了找到并克隆出影响长爪沙鼠不同willis血管类型表达的基因,建立包含各种血管类型的全基因组文库。方法选取清洁级长爪沙鼠96只,解剖后剥离不同willis血管类型,分别提取DNA后混合。选用Copy Control Fosmid Library Kit和p CC1FOS载体构建文库,并对文库进拷贝数、重组片段长度及重组率进行鉴定。结果成功建立了长爪沙鼠不同willis血管类型的全基因组文库,文库容量为1700个拷贝数,插入重组片段长度约为36 kb,重组率为93%。结论建立了不同willis血管类型的长爪沙鼠全基因组文库,为后续基因克隆及筛选等研究打下良好基础。Objective To find out and clone the genes that can influence different willis circle in Meriones unguiculatus, the genomic library has been made. Methods 96 clean Medones unguiculatus were dissected according to observe the differences of willis circle, and the mixed DNA was extracted from different blood vessels. The genomic library construction has been done by using pCC1FOS vector, following by CopyControl Fosmid Lib manual. The copy numbers, recombined segment size and recombination fraction of the library were measured. Results Genomic library of different Willis circle in Meriones unguiculatus was successfully made. The capacity of the library was 1700 copies. The segment size was 36kb, aud the recombination fraction was 93%. Conclusion The library was the good beginning for the following steps which were gene cloning and genescreen.

关 键 词：长爪沙鼠 脑底动脉环 全基因组 文库 

分 类 号：R332[医药卫生—人体生理学]