橙皮素对脂多糖诱导H9C2心肌细胞活性氧水平的影响  

Effect of Hesperetin on the Level of Reactive Oxygen Species in H9C2 Cardiomyocytes which Induced by Lipopolysaccharides

作　　者：邓伟[1,2] 刘源[1,2] 陈昌贵[1,2] 魏丽[2,3] 唐其柱[1,2] 

机构地区：[1]武汉大学人民医院心血管内科,430060 [2]武汉大学心血管病研究所,430060 [3]武汉大学人民医院儿科,430060

出　　处：《医学研究杂志》2014年第11期36-38,共3页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81300104);教育部博士点基金资助项目(20130141120042);湖北省自然科学基金资助项目(2013CFB303)

摘　　要：目的探讨橙皮素对脂多糖(lipopolysaccharides,LPS)诱导H9C2心肌细胞活性氧(reactive oxygen species,ROS)水平的影响。方法使用不同浓度橙皮素(0.0625、0.125、0.25μmol/L)和LPS(1μg/ml)共同孵育H9C2心肌细胞120min,DCFHDA探针检测H9C2细胞ROS水平,荧光显微镜定性观察以及流式细胞仪定量检测各组细胞荧光强度,以观察不同浓度橙皮素对LPS诱导H9C2心肌细胞产生ROS的影响。选择0.25μmol/L橙皮素和LPS(1μg/ml)共同孵育H9C2心肌细胞30、60、120min,观察橙皮素对LPS诱导H9C2心肌细胞产生ROS的时间相关性。结果橙皮素干预可以浓度依赖性的缓解LPS诱导H9C2心肌细胞ROS产生;在60、120min,橙皮素能够抑制LPS诱导H9C2心肌细胞ROS产生。结论橙皮素能够抑制LPS诱导的H9C2心肌细胞ROS产生,从而降低心肌细胞氧化应激水平,其有可能成为心肌保护新的潜在药物。Objective To investigate the effect of hesperetin on the level of reactive oxygen species（ROS） in H9C2 cardiomyocytes which induced by lipopolysaccharides（LPS）.Methods The embryonic rat heart-drived H9C2 cells were coincubated with different concentrations of hesperetin（0.0625,0.125,0.25 μmol/L）and LPS（1 μg/ml）for 120min.And then H9C2 cells were coincubated with hesperetin（0.25μmol/L）and LPS（1μg/ml）for different time（30,60,120min）.Results We found that H9C2 cells treated with hesperetin could be protected from LPS-induced ROS production.The decreasing ROS levels in LPS-stimulated cells were concentration-dependent and time-related by hesperetin treatment.Conclusion Hesperetin can reduce the ROS production and attenuate oxidative stress in H9C2 cardiomyocytes which induced by LPS.

关 键 词：橙皮素 脂多糖 H9C2心肌细胞 活性氧 

分 类 号：R5[医药卫生—内科学]