CEA多表位融合蛋白在胃肠道肿瘤血清学诊断中的研究价值  

Research Value of CEA Multi-epitope Fusion Protein in the Serological Diagnosis of Gastrointestinal Tumors

作　　者：王鹏飞[1] 朱茜[1] 张丽芳[2] 朱冠保[1] 

机构地区：[1]温州医科大学附属第一医院胃肠外科,325000 [2]温州医科大学微生物与免疫教研室,325000

出　　处：《医学研究杂志》2014年第11期54-56,共3页Journal of Medical Research

基　　金：浙江省自然科学基金资助项目(Y2100660)

摘　　要：目的以CEA多表位融合蛋白为抗原,即ozlf-86/87融合蛋白,检测胃肠道肿瘤患者血清中抗CEA抗体,鉴定融合蛋白的抗原性,为融合蛋白作为诊断抗原提供依据,为下一步动物免疫反应实验奠定基础。方法以CEA多表位融合蛋白作为诊断抗原,采用间接ELISA法,分别对收集的39例胃癌患者血清、25例结肠癌患者血清、30例慢性胃炎患者血清和30例健康对照者的血清中的抗CEA抗体进行检测,用方差检验分析胃结肠癌患者实验组和慢性胃炎、健康患者对照组血清抗体效价之间差异性。结果胃癌和结肠癌实验组与对照组血清抗体效价之间差异均有统计学意义(P<0.01)。对胃癌患者和结肠癌患者血清抗体的诊断敏感度分别为35.9%和44.0%,结论本实验通过工程菌表达纯化的CEA多表位融合蛋白具有很好的抗原性,对用于血清学诊断的研究有一定的可行性,对制备ELISA血清检测试剂盒提供了理论基础,为下一步免疫反应及抗CEA抗体的制备奠定基础。Objective Multiple-epitope fusion protein CEA,also named ozlf-86/87 fusion protein,was detected in the patients with gastrointestinal cancer in order to provide the basis of the fusion protein as a diagnostic antigen and lay the foundation for further animal experiments immune response.Methods The serumal anti-CEA antibodies were detected by a diagnostic antigen,multiple-epitope fusion Protein CEA,with indirect ELISA in 39 cases of gastric cancer patients,25 patients with colon cancer,30 patients with chronic gastritis and 30 healthy controls,respectively.The difference of serumal antibody titers between cohorts was analysed by ANOVA.Results There were significant differences of serumal antibody titers （P ＜ 0.01） between the stomach and colon cancer in the experimental group and the control group.The diagnostic sensitivity of serumal antibodies in gastric cancer and colon cancer patients were 35.9％ and 44.0％,respectively.Conlusion Purified by engineered bacteria,multiple-epitope fusion Protein CEA emerges a promising antigenic expression and the feasibility of serological diagnosis study.It also provides the theoretical basis for the manufacture of serum ELISA test kit and the foundation of anti-CEA antibody producing and relating immune response.

关 键 词：CEA 融合蛋白 抗CEA抗体 胃肠道肿瘤 

分 类 号：R604[医药卫生—外科学]