灵芝多糖对辐射损伤小鼠的防护作用  

Radioprotective effect of Ganoderma lucidum polysaccharides on irradiated mice

作　　者：丁妍[1] 周向毅[2] 崔莉[3] 陈海江 张勇妹[1] 郭贵宾[1] 孙锐[1] 陈邦元[1] 

机构地区：[1]解放军第三五九医院药械科,镇江212001 [2]解放军第三五九医院肿瘤科,镇江212001 [3]江苏省中医药研究院国家中医药管理局中药释药系统重点研究室,南京210028 [4]南京市红十字医院中医科,南京210002

出　　处：《医学研究生学报》2014年第11期1152-1155,共4页Journal of Medical Postgraduates

基　　金：南京军区医学科技创新课题(12 MA042)

摘　　要：目的辐射可引起多种组织器官的损伤。文中旨在探讨灵芝多糖对受60Coγ射线损伤小鼠的辐射防护作用,为灵芝多糖的临床应用提供实验依据。方法采用大剂量60Coγ射线全身照射雌性小鼠,制成放射损伤动物模型。小鼠按随机数字表法分为正常对照组、灌胃对照组、辐射对照组、灵芝多糖高剂量保护组、灵芝多糖低剂量保护组。灵芝多糖保护组小鼠在照射前3 d及照射后,以不同剂量的灵芝多糖连续灌胃14 d。观察受致死剂量60Coγ射线照射小鼠的30 d存活率及生存时间长短。同时,检测用药后外周血指标、脾指数、血清超氧化物歧化酶(superoxide dismutase,SOD)活性的变化。结果实验结束时,灵芝多糖组小鼠的30 d生存期[(23.7±6.8)、(28.3±5.9)d]显著高于辐射对照组[(18.3±5.4)d],差异有统计学意义(P<0.01);灵芝多糖组小鼠的外周血WBC[(2.89±0.67)×109/L、(2.77±0.72)×109/L]显著高于辐射对照组[(2.54±0.63)×109/L],差异有统计学意义(P<0.05),且灵芝多糖组PLT数[(487.20±165.77)×109/L、(716.36±223.44)×109/L]显著高于辐射对照组[(387.45±154.22)×109/L],差异有统计学意义(P<0.05);灵芝多糖高剂量组脾指数显著高于辐射对照组[(0.005 4±0.002 1)vs(0.003 2±0.000 7),P<0.05)];灵芝多糖组小鼠的血清SOD含量[(401.27±35.26)、(369.02±29.70)U/m L]明显高于辐射对照组[(311.32±23.71)U/m L],差异有统计学意义(P<0.05)结论灵芝多糖具有较强的抗辐射作用,能显著提高受致死剂量60Coγ射线照射小鼠的存活率,降低辐射对小鼠外周血WBC和PLT的损伤作用,并提高SOD活性,其具体作用机制有待进一步深入研究。Objective Radiation can cause multiple damages to tissues and organs.This study aimed to explore the protec-tive effect of Ganoderma lucidum polysaccharides （ GLPs） against the 60 Co-γray radiation injury in mice and provide an experimental basis for the clinical use of GLPs. Methods One hundred mice were randomly divided into five groups of equal number： normal control, gavage control, radiation control, high-dose GLPs, and low-dose GLPs.Models of radiation injury were made in the mice by whole-body exposure to 60 Co-γrays.Three days before and after mod-eling, the animals in the high-dose and low-dose GLPs groups were given GLPs intragastrically at the dose of 100 and 50 mg/kg respec-tively, once daily for 14 days.Then the 30 day survival rate and sur-vival time of the model mice were recorded and the changes in the pe-ripheral blood index, spleen index, and serum superoxide dismutase（ SOD） activity were observed. Results GLPs significantly increased the 30-day survival rate and the mean survival time of the mouse models （P〈0.05）, decreased the reduction of WBC count in the peripheral blood, and shortened the time of WBC restoration （ P〈0.05 ） .Furthermore, GLPs obviously improved the spleen index and SOD activity of the Co-γray irradiated animals. Conclusion GLPs, with a significant anti-radiation effect, can effectively raise the survival rate of the mice exposed to a lethal dose of 60 Co-γrays, reduce radiation injury to WBC and platelets, and increase the activity of SOD in irradiated mice.

关 键 词：灵芝多糖 辐射损伤 辐射防护 

分 类 号：R979.6[医药卫生—药品]