参麦注射液足三里穴位注射对大鼠SIRS时肿瘤坏死因子的影响  

Effects of Shenmai Injection Zusanli Acupoint Injection on Rats SIRS Inflammatory Factor TNF-α

作　　者：兰菲[1] 傅天啸[1] 华晨[1] 周剑[1] 任大为[1] 傅永清[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《云南中医学院学报》2014年第5期9-12,共4页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：浙江中医药管理局基金项目(2012ZA043)

摘　　要：目的观察参麦注射液足三里穴位注射对大鼠全身炎症反应综合征(systemic inflammatory response syndrome,SIRS)时肿瘤坏死因子-α(TNF-α)的影响。方法建立大鼠SIRS模型。40只SD大鼠随机分成空白对照组、针灸治疗组、参麦注射组和生理盐水组,每组各10只。造模成功1h后观察各组大鼠的一般情况;经5d治疗后,收集样本,检测血常规(白细胞计数WBC)、动脉血气分析(PCO2、PO2)、血清肿瘤坏死因子α(TNF-α)水平。结果参麦注射组WBC、TNF-α水平显著低于其余3组(P<0.05)。参麦注射组PO2水平显著高于空白对照组(P<0.05),参麦注射组PCO2水平显著低于空白对照组(P<0.05)。结论参麦注射液足三里穴位注射治疗大鼠SIRS可减轻炎症反应,抑制炎症因子,保护肺功能。Objective To observe the protective effects of Shenmai injection in Zusanli acupoint injection on rats systemic inflammatory response syndrome inflammatory factor TNF-α. Methods To establish a comprehensive model of systemic inflammatory response syndrome in rats. Forty SD rats were randomly divided into four groups （n=10）, including Vacuity-contrast group（VC group）、 Acupuncture in Zusanli group （AZ group）、 Shenmai injection in Zusanli acupoint injection group （SZ group） and Physiological saline Zusanli point injection group （PZ group）. To observed the general condition of rats when modeling success after 1h. After 5D treatment the rats were sacrificed, collect samples, detection of blood（white blood cell count, WBC）;arterial blood gas analysis （PCO2, PO2）; serum tumor necrosis factor alpha （TNF-α） level. Results The WBC、 TNF-α levels of the SZ group were significantly lower than the other three group （P〈0.05）. The PCO2 levels of the SZ group were significantly lower than VC group （P〈0.05）. The PO2 levels of the SZ group were significantly higher than VC group （P〈0.05）. Conclusion Shenmai injection in Zusanli acupoint injection for treatment of SIRS in rats may reduce the inflammation response, inhibit inflammatory factors, to protect lung function.

关 键 词：大鼠 全身炎症反应综合征 参麦注射液 足三里穴位注射 TNF-Α 

分 类 号：R285.5[医药卫生—中药学]